Get the free ONE-STEP PHAGE GROWTH CURVE I OBJECTIVES II INTRODUCTION - sas upenn

Microbiology BIOL 275 ONES TEP PHAGE GROWTH CURVE I. OBJECTIVES To construct the growth curve of a phage from laboratory data and determine its burst size. To identify the phases of a phage growth

How to fill out one-step phage growth curve

How to fill out a one-step phage growth curve:

1. Prepare the culture: Start by inoculating a bacterial culture with a known concentration of phage. Make sure to mix the culture well to distribute the phage evenly.
2. Incubation: Allow the phage and bacteria to incubate together for a specified period of time, typically at a specific temperature and shaking conditions. This incubation period allows for the phage to infect and replicate within the bacterial culture.
3. Sampling: During the incubation period, take regular samples at specific time intervals. These samples will be used to determine the phage concentration at different time points throughout the growth curve.
4. Dilution and Plating: For each sample, perform serial dilutions to reduce the phage concentration and make it easier to count individual phage particles. Plate the diluted samples onto agar plates seeded with a suitable bacterial host to allow for the formation of plaques.
5. Counting and Calculation: After incubation, count the number of plaques on each plate. The plaque-forming units (PFUs) represent the number of active phage particles in the original sample. Use these counts to calculate the phage concentration at each time point.

Who needs one-step phage growth curve?

1. Researchers studying bacteriophages: Phage growth curves are commonly used in research to understand the replication and growth dynamics of bacteriophages. By filling out a one-step growth curve, researchers can gain insights into the viral life cycle, burst size, latent period, and other important parameters.
2. Phage biotechnologists: Those working in the field of phage biotechnology may need to fill out one-step phage growth curves to optimize phage production for various applications, such as phage therapy or biocontrol. Understanding the growth characteristics of phages can help in designing efficient production processes.
3. Quality control laboratories: Some laboratories or facilities may use phages for quality control purposes, such as testing the efficiency of disinfection procedures. By performing one-step phage growth curves, these labs can ensure the viability and effectiveness of their phages before using them for testing or application.

In conclusion, filling out a one-step phage growth curve involves preparing the culture, incubation, sampling, dilution and plating, and counting and calculation of phage concentrations at different time points. This technique is commonly used by researchers, phage biotechnologists, and quality control laboratories.

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