Get the free Graduated and Micropipettes - Sacramento City College - scc losrios

Experiment 1: Volumetric Measurement; Using Micropipets and Graduated Pipes Adapted from the CSS Biochemistry (Chem 162) Lab Manual, Fall 2002. And The Science Learning Center at the University of

How to fill out graduated and micropipettes

How to fill out graduated and micropipettes:

1. Ensure that the pipette is clean and free from any residue. This can be done by rinsing with water or a suitable cleaning solution.
2. Prepare the liquid sample that needs to be transferred. This could be a solution, a reagent, or any other liquid substance.
3. Before filling the pipette, set the desired volume by adjusting the appropriate dial or plunger on the pipette. Graduated pipettes usually have markings along the stem indicating different volume measurements, whereas micropipettes typically have a digital display or a dial for setting the volume.
4. To fill a graduated pipette, use a dedicated pipette filler or a bulb and attach it to the top end of the pipette. Immerse the tip of the pipette into the liquid sample and slowly release the bulb or pipette filler to create a vacuum. As the vacuum is released, the liquid will be drawn up into the pipette.
5. For micropipettes, select the appropriate pipette tip size and attach it securely to the end of the pipette. Depress the plunger to the first stop position and immerse the tip into the liquid sample. Slowly release the plunger to draw up the required volume into the micropipette.
6. Once the desired volume has been drawn into the pipette, remove it from the liquid source and ensure that there are no air bubbles present in the tip. If there are bubbles, gently tap the pipette or release the liquid and refill to eliminate them.
7. To transfer the liquid, place the tip of the pipette into the desired container or vessel. Graduated pipettes often have a rubber bulb release mechanism, whereas micropipettes have a plunger release button. Release the liquid by either releasing pressure on the bulb or by fully pressing the plunger in one swift motion.
8. After transferring the liquid, release the pressure on the bulb or plunger. Graduated pipettes can be rinsed with a suitable rinsing solution, while micropipettes should be wiped with a lint-free tissue or wiped with an ethanol solution to remove any liquid residue.
9. Graduated and micropipettes are essential tools for researchers, scientists, laboratory technicians, and healthcare professionals who need to accurately measure and transfer small volumes of liquids. They are commonly used in various scientific fields such as chemistry, biology, microbiology, biochemistry, and clinical diagnostics.

Who needs graduated and micropipettes:

1. Researchers: Graduated and micropipettes are indispensable tools for researchers who conduct experiments and studies that require precise volume measurements and liquid transfers. They enable accurate and reproducible results in fields such as pharmaceutical research, biotechnology, genetics, and more.
2. Scientists: Scientists working in laboratories, whether in academia or industry, often rely on graduated and micropipettes to handle small liquid volumes in their experiments. These tools allow them to dispense reagents, samples, or specific amounts of chemicals with high precision.
3. Laboratory technicians: In laboratory settings such as medical labs, diagnostic labs, or quality control labs, technicians use graduated and micropipettes extensively for sample preparation, drug formulation, analytical testing, and quality assurance procedures.
4. Healthcare professionals: Graduated and micropipettes are also used by healthcare professionals in settings such as hospitals, clinics, and research facilities. These tools are vital for conducting medical tests, analyzing bodily fluids, preparing medications, and carrying out various diagnostic procedures.

For pdfFiller’s FAQs

What is graduated and micropipettes?

Graduated pipettes are used for measuring various volumes of liquids accurately. Micropipettes are used for transferring small, precise volumes of liquid.

Who is required to file graduated and micropipettes?

Laboratories, pharmaceutical companies, research facilities, and other scientific institutions are required to file graduated and micropipettes.

How to fill out graduated and micropipettes?

Graduated pipettes should be filled by drawing the liquid into the pipette to the desired volume, while micropipettes usually require the use of a dial or knob to set the desired volume.

What is the purpose of graduated and micropipettes?

The purpose of graduated and micropipettes is to accurately measure and transfer liquid volumes in various scientific experiments and processes.

What information must be reported on graduated and micropipettes?

The information reported on graduated and micropipettes typically includes the volume being measured, the liquid being transferred, and any specific markings or calibrations on the pipette.

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