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AMYLASE LAB STUDENT HANDOUT..............................pages 113 TEACHER REFERENCE PAGES...........pages 1421Factors Affecting the Activity of Salivary Amylase STUDENT HANDOUT EQUIPMENT Vortex mixers,

How to fill out amylase lab

How to fill out amylase lab:

1. Gather all the necessary materials and equipment for the lab, including test tubes, amylase solution, substrate (starch solution), water bath, stopwatches, pipettes, and a colorimeter.
2. Label each test tube with a unique identifier to keep track of the different samples.
3. Prepare the amylase solution by following the provided instructions or guidelines. Ensure that the solution is properly diluted to the desired concentration.
4. Fill each test tube with a specific amount of the amylase solution, as stated in the lab instructions.
5. Prepare the substrate solution by mixing the provided starch solution with water in the required proportions. Make sure the solution is homogeneous.
6. Fill another set of test tubes with the substrate solution. Again, follow the instructions regarding the volume and concentration.
7. Initiate the reaction by adding the amylase solution to the test tubes containing the substrate solution. Make sure to perform this step simultaneously for all the samples to maintain consistency.
8. Start the stopwatch as soon as the amylase and substrate solutions are mixed. Record the time accurately.
9. Place the test tubes in a water bath set at a specific temperature, usually mentioned in the lab guidelines. The water bath ensures the maintenance of a constant temperature during the reaction.
10. Allow the reaction to proceed for the specified duration, usually mentioned in the lab instructions or until the reaction reaches a predefined endpoint.
11. After the reaction time has elapsed, remove the test tubes from the water bath and measure the absorbance of each sample using a colorimeter. Follow the colorimeter's instructions to obtain accurate readings.
12. Record the absorbance values for each sample in a table or lab report sheet provided, along with the corresponding reaction time.
13. Clean and dispose of all the used materials following the lab's safety protocols and guidelines.

Who needs amylase lab:

1. Students studying biochemical processes or enzyme kinetics may need an amylase lab to gain practical knowledge and reinforce theoretical concepts.
2. Researchers or scientists conducting experiments or research related to factors affecting amylase activity or analyzing enzyme kinetics might require an amylase lab.
3. Healthcare professionals, particularly in the field of clinical chemistry, might use amylase lab tests to diagnose and monitor certain medical conditions related to the pancreas or salivary glands.

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