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Europaisches Patent amt (19) European Patent Office European DES brevet seen EP 0 826 964 A2 E U R O P E A N PATENT A P P L I C A T I O N (12) (43) Date of publication: 04.03.1998 Bulletin 1998/10
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How to fill out determination of protein
How to fill out determination of protein:
01
Begin by gathering all the necessary materials and reagents for the protein determination assay. This may include protein standard solutions, assay buffers, enzyme solutions, colorimetric reagents, and a spectrophotometer.
02
Prepare the protein samples for analysis. This may involve extracting the protein from a biological sample using techniques like homogenization, centrifugation, or precipitation. Ensure that the protein concentration is within the linear range of the assay.
03
Prepare the protein standards to generate a standard curve. Dilute the protein standard solutions to obtain a series of known concentrations. It is important to have standards covering a range of concentrations to accurately determine the protein concentration in the unknown samples.
04
Set up the assay plate or cuvette. Add the appropriate volumes of the protein samples and standards into the wells or cuvettes following the assay protocol. Ensure that each sample and standard is added to its respective well.
05
Add the necessary reagents to initiate the protein determination reaction. This may involve adding an enzyme solution or colorimetric reagent to the wells or cuvettes. Follow the assay protocol for the specific reagents and incubation times.
06
Measure the absorbance of the samples and standards at the appropriate wavelength using a spectrophotometer. This will provide the data necessary for calculating the protein concentration.
07
Plot the absorbance values of the standards on a graph against their known concentrations. Use this standard curve to calculate the protein concentration of the unknown samples based on their corresponding absorbance values.
08
Record the protein concentrations for each sample in the determination of protein form or spreadsheet. Make sure the data is accurate and correctly matched to the corresponding sample.
Who needs determination of protein:
01
Researchers in the field of biochemistry and molecular biology often require protein determination to quantify the amount of protein present in biological samples. This allows them to study protein-protein interactions, protein expression levels, and protein purification processes.
02
Clinical laboratories may perform protein determination as part of diagnostic tests. Protein levels can be indicative of certain diseases or conditions, and determining protein concentration can assist in diagnosis and monitoring of patients.
03
Industries involved in protein production, such as biopharmaceutical companies, may perform protein determination to ensure product quality and consistency. By accurately measuring protein concentration, they can optimize production processes and meet regulatory requirements.
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What is determination of protein?
The determination of protein is the process of analyzing and quantifying the amount of protein present in a sample.
Who is required to file determination of protein?
Research institutions, food manufacturers, and biotech companies are typically required to file determination of protein.
How to fill out determination of protein?
Determining protein content usually involves using laboratory techniques such as spectroscopy, chromatography, and electrophoresis.
What is the purpose of determination of protein?
The purpose of determination of protein is to assess the nutritional content or purity of a product, or to conduct research on protein structure and function.
What information must be reported on determination of protein?
The determination of protein report typically includes the method used for analysis, the results obtained, and any relevant sample information.
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