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(19) J mi m i ii m mi i ii ii ii i ii European Patent Office European DES brevets (11) EP 0 790 31 1 A 1 E U R O P E A N PATENT A P P L I C A T I O N (12) (43) Date of publication: 20.08.1997 Bulletin
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How to fill out microspore-specific promoter from tobacco
Instructions for filling out microspore-specific promoter from tobacco:
01
Start by obtaining the necessary materials for the experiment, such as tobacco plants and tools for genetic manipulation.
02
Prepare the tobacco plants by growing them under controlled conditions, ensuring they are healthy and suitable for experimentation.
03
Isolate the desired microspore-specific promoter from the tobacco plant. This can be done using molecular biology techniques such as PCR or gene cloning.
04
Once the microspore-specific promoter has been successfully isolated, verify its identity and purity using genetic analysis methods.
05
Design the experimental setup based on the specific objective you aim to achieve with the microspore-specific promoter. This could involve introducing the promoter into other plants or organisms, or studying its activity in more detail.
06
Choose the appropriate method for introducing the microspore-specific promoter into the target plants or organisms. This could involve genetic transformation techniques, such as Agrobacterium-mediated transformation or particle bombardment.
07
Perform the necessary genetic manipulations to introduce the microspore-specific promoter into the target plants or organisms. Follow established protocols and ensure proper documentation of the experimental procedure.
08
Cultivate the transformed plants or organisms under suitable growth conditions, allowing them to express the microspore-specific promoter.
09
Monitor the activity and effects of the microspore-specific promoter in the transformed plants or organisms. This could involve observing changes in gene expression, phenotype, or other relevant characteristics.
10
Evaluate the results obtained and analyze any data collected during the experiment. Draw conclusions and potentially repeat the experiment if needed to validate the findings.
Who needs microspore-specific promoter from tobacco?
01
Researchers or scientists studying plant development and reproductive biology may require microspore-specific promoters from tobacco for their experiments.
02
Genetic engineers and biotechnologists interested in manipulating gene expression in plants could benefit from using microspore-specific promoters from tobacco.
03
Plant breeders or agricultural professionals aiming to enhance crop production or improve plant traits may find microspore-specific promoters useful in their work.
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What is microspore-specific promoter from tobacco?
The microspore-specific promoter from tobacco is a regulatory element that controls the expression of genes specifically in microspores of tobacco plants.
Who is required to file microspore-specific promoter from tobacco?
Researchers, scientists, or companies working with genetically modified tobacco plants are required to file the microspore-specific promoter from tobacco.
How to fill out microspore-specific promoter from tobacco?
The microspore-specific promoter from tobacco can be filled out by providing detailed information about the promoter sequence, genetic modifications, and intended use of the promoter.
What is the purpose of microspore-specific promoter from tobacco?
The purpose of the microspore-specific promoter from tobacco is to regulate the expression of genes specifically in microspores, resulting in targeted genetic modifications or gene expression.
What information must be reported on microspore-specific promoter from tobacco?
The information to be reported on the microspore-specific promoter from tobacco includes the nucleotide sequence of the promoter, the gene(s) it controls, and any associated regulatory elements.
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