Get the free Vector and mammalian cell line having improved productivity - European Patent Office...

S OIL *S Ill IN III Ml II III Mil II I II European Patent Office European DES brevets (11) EP 0 874 057 A2 E U R O P E A N PATENT A P P L I C A T I O N (12) (43) Date of publication: 28.10.1998 Bulletin

How to fill out vector and mammalian cell

How to fill out vector and mammalian cell:

1. First, gather all the necessary materials and reagents needed for the process.
2. Prepare the vector DNA by isolating it from a suitable source and purifying it. This can be done using various methods such as plasmid extraction kits or column purification techniques.
3. Next, select the appropriate restriction enzymes and digest the vector DNA. This step helps to create compatible ends for the subsequent ligation reaction.
4. Similarly, obtain the desired DNA fragment to be inserted into the vector. This fragment can be obtained through PCR amplification or gene synthesis techniques.
5. Digest the DNA fragment using the same restriction enzymes used for the vector digestion, ensuring compatibility for ligation.
6. Combine the digested vector DNA and the DNA fragment in a ligation reaction. This reaction is performed using a DNA ligase enzyme, which catalyzes the joining of the two DNA molecules.
7. Transform the ligated DNA into competent bacterial cells. This can be achieved through various methods such as heat shock or electroporation. The transformed cells will allow for replication and amplification of the recombinant DNA.
8. Select the transformed bacteria using antibiotic selection, if applicable, to ensure that only cells containing the desired vector with the inserted DNA fragment survive.
9. Once the desired clone containing the recombinant vector is identified, isolate the plasmid DNA from the bacteria and confirm the presence of the inserted DNA fragment using techniques such as PCR or restriction enzyme digestion.
10. Finally, the purified plasmid with the inserted DNA fragment can be used to transfect or transduce mammalian cells, depending on the specific experimental requirements.

Who needs vector and mammalian cell?

1. Researchers involved in genetic engineering and molecular biology often require vectors and mammalian cells for various purposes.
2. Vector systems are widely used in molecular cloning to introduce foreign DNA into cells, allowing for the expression and study of specific genes.
3. Mammalian cells are particularly relevant for studies involving human or animal gene expression, protein production, drug development, and various disease models.
4. Biotechnology and pharmaceutical companies use vectors and mammalian cells to produce recombinant proteins, vaccines, and therapeutics.
5. Academic institutions, research laboratories, and medical facilities also rely on vectors and mammalian cells for a wide range of experiments and scientific investigations.

For pdfFiller’s FAQs

What is vector and mammalian cell?

Vector is a DNA molecule used as a vehicle to transfer foreign genetic material into another cell. Mammalian cell is a cell derived from a mammal, such as a human cell or a mouse cell.

Who is required to file vector and mammalian cell?

Researchers and scientists working with genetic materials and cell lines are required to file vector and mammalian cell.

How to fill out vector and mammalian cell?

Vector and mammalian cell forms are typically filled out online or through a designated regulatory platform provided by the relevant authorities.

What is the purpose of vector and mammalian cell?

The purpose of filing vector and mammalian cell is to ensure transparency and regulatory compliance in the use of genetic materials and cell lines in research and biotechnological applications.

What information must be reported on vector and mammalian cell?

The information to be reported on vector and mammalian cell includes details of the genetic material used, the source of the mammalian cell line, the purpose of the research, and relevant safety precautions.

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