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Nova Lisa TM Toxoplasma Gandhi IGM -capture ELISA 0483 Enzyme immunoassay for the qualitative determination of Pre-class antibodies against Toxoplasma Gandhi in human serum or plasma Enzymimmunoassay
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How to fill out igm capture elisa
How to fill out igm capture elisa:
01
Gather all the necessary materials and reagents needed for the test including the capture antibody, detection antibody, buffers, and controls.
02
Prepare the microplate by coating it with the capture antibody. Add a specific amount of the antibody to each well and incubate at a particular temperature for a set period of time.
03
Wash the microplate multiple times to remove any unbound capture antibody.
04
Block the remaining protein binding sites on the microplate by adding a blocking buffer and incubating for a designated time.
05
Wash the microplate again to remove any excess blocking buffer.
06
Add the patient's sample (serum or plasma) to the microplate wells and incubate to allow the capture of any specific igM antibodies present.
07
Wash the microplate to remove any unbound material.
08
Add the detection antibody to the wells and incubate to allow the binding of the detection antibodies to the captured antigens.
09
Wash the microplate to remove any unbound detection antibody.
10
Add a substrate solution that will produce a detectable signal in the presence of the bound detection antibody.
11
Incubate the microplate for a specific time to allow the substrate reaction to occur.
12
Stop the reaction by adding a stop solution.
13
Measure the absorbance or fluorescence of each well in the microplate using a microplate reader.
14
Analyze the obtained results, comparing the absorbance or fluorescence values of the patient's sample to the controls.
Who needs igm capture elisa:
01
Individuals who have been recently exposed to an infectious agent and are suspected of having an acute infection.
02
Researchers studying the immune response to certain diseases or pathogens.
03
Clinicians or healthcare professionals evaluating the progression or treatment efficacy of certain diseases.
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