Get the free SUBJECT HEMATOXYLIN AND EOSIN STAINING I PRINCIPLE - ukhealthcare uky

University of Kentucky Market Cancer Center Biospecimen Core Program BCP H.2 113A, Combs Building, 800 Rose Street.; Lexington, KY 40536; pH: (859) 2574717; pH: (859) 3232618; fax: (859) 2573757 1092012-Page

How to fill out subject hematoxylin and eosin

How to fill out subject hematoxylin and eosin:

1. Begin by preparing the hematoxylin solution according to the manufacturer's instructions. This usually involves diluting a concentrated solution with a specific volume of distilled water. Remember to wear proper protective equipment, such as gloves and goggles, when handling chemicals.
2. Next, prepare the tissue sections or slides that you want to stain with hematoxylin and eosin. Ensure that the sections are clean and properly mounted on the slides. You can use fresh or fixed tissue samples, depending on your experiment or study.
3. Before applying the hematoxylin solution, it is important to deparaffinize and rehydrate the tissue sections. This process typically involves immersing the slides in xylene or a xylene substitute to remove the paraffin, followed by a series of alcohol washes to rehydrate the tissue.
4. Once the tissue sections are ready, carefully apply the hematoxylin solution to cover the specimens entirely. The staining time may vary depending on the manufacturer's instructions, the desired intensity of staining, and the type of tissue being used. Typically, this ranges from a few minutes to overnight incubation.
5. After staining with hematoxylin, rinse the slides with distilled water to remove excess stain. Be gentle to avoid damage or loss of tissue sections.
6. The next step is to apply the eosin solution. Eosin is a counterstain that provides contrast to the hematoxylin-stained nuclei. Like with hematoxylin, prepare the eosin solution following the manufacturer's instructions.
7. Apply the eosin solution onto the tissue sections for a specified period, usually ranging from a few seconds to a couple of minutes. This will depend on the desired intensity of the counterstain. Rinse the slides with distilled water once again to remove excess eosin.
8. After rinsing, dehydrate the tissue sections using a series of alcohol washes, starting from lower concentrations (e.g., 70% ethanol) to higher concentrations (e.g., 100% ethanol). This process helps to remove excess water and prepare the slides for mounting.
9. Finally, mount the slides with a suitable mounting medium, such as a synthetic resin or a xylene-based mounting medium. This will preserve the stained tissue sections and allow for microscopic examination.

Who needs subject hematoxylin and eosin:

Hematoxylin and eosin (H&E) staining is widely used in histology and pathology laboratories. It is essential for researchers, medical professionals, pathologists, and histotechnologists who study tissues and diagnose diseases. H&E staining provides crucial information about tissue architecture, cellular morphology, and abnormalities. It helps in identifying various diseases, such as cancer, infections, and inflammatory conditions. From research laboratories to clinical settings, anyone working with tissue samples or involved in pathological analysis can benefit from using hematoxylin and eosin staining.

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