Get the free N-Terminal Edman Sequencing - Uni-GiessenDE - uni-giessen

Per Email sender Formula drunken JustusLiebigUniversity Giessen, Faculty of Medicine, Institute of Biochemistry, Friedrichstrasse 24, 35392 Giessen http://www.unigiessen.de/cms/fbz/fb11/institute/biochemie/forschung/proteinanalytik

How to fill out n-terminal edman sequencing

How to fill out n-terminal Edman sequencing:

1. Begin by preparing a sample containing the protein you want to sequence. This can usually be obtained through protein purification techniques.
2. Once you have your sample, immobilize it onto a solid support such as a membrane or a resin. This allows for easier analysis during the sequencing process.
3. The next step is to perform the actual Edman degradation reaction. This involves treating the sample with phenylisothiocyanate (PITC), which selectively reacts with the amino terminus of the protein.
4. After the reaction, remove any excess PITC and then cleave the tagged amino terminus from the protein using a cleavage reagent, often trifluoroacetic acid (TFA).
5. Once the amino terminus is released, it can be analyzed using techniques such as high-performance liquid chromatography (HPLC) or mass spectrometry to determine the identity of the amino acid.
6. Repeat the steps above iteratively to sequence the protein in a stepwise manner until the desired length is reached.

Who needs n-terminal Edman sequencing:

1. Researchers and scientists studying proteins often utilize n-terminal Edman sequencing to determine the primary structure of a protein, particularly for smaller proteins or peptides.
2. This technique is particularly useful in protein identification and characterization, especially when combined with other analytical methods such as mass spectrometry.
3. N-terminal Edman sequencing is also commonly employed in studying protein modifications, identifying post-translational modifications (PTMs) that occur at or near the protein's amino terminus.

Note: These are general guidelines for n-terminal Edman sequencing. It's important to consult specific protocols and literature for detailed procedures and variations depending on the specific protein or research objectives.

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What is n-terminal edman sequencing?

N-terminal Edman sequencing is a method used to determine the amino acid sequence of a protein.

Who is required to file n-terminal edman sequencing?

Scientists and researchers studying proteins are typically required to perform n-terminal Edman sequencing.

How to fill out n-terminal edman sequencing?

To fill out n-terminal Edman sequencing, a protein sample is subjected to a series of chemical reactions to identify the amino acid sequence.

What is the purpose of n-terminal edman sequencing?

The purpose of n-terminal Edman sequencing is to determine the order of amino acids in a protein molecule.

What information must be reported on n-terminal edman sequencing?

The report must include the amino acid sequence obtained from the protein sample.