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31 Experiment 3 SPECTROPHOTOMETRY DETERMINATION OF DISSOLVED IRON REFERENCE: Text, Chapter 18, Sect. 181 184; (also see similar lab at www.whfreeman.com/qca experiment 19). INTRODUCTION Iron can exist
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How to fill out experiment 3 spectrophotometric determination
How to fill out experiment 3 spectrophotometric determination:
01
Start by preparing the necessary materials and equipment for the experiment, including a spectrophotometer, cuvettes, a standard solution, and the sample to be analyzed.
02
Calibrate the spectrophotometer by adjusting the wavelength and setting the absorbance to zero with the blank solution. This ensures accurate readings for the sample.
03
Prepare the standard solution by diluting a concentrated stock solution to the desired concentration. This solution will be used to create a calibration curve for determining the concentration of the unknown sample.
04
Measure the absorbance of the standard solution using the spectrophotometer. Record the absorbance values at different concentrations of the standard solution.
05
Plot the absorbance values against the corresponding concentrations of the standard solution on a graph to create a calibration curve. This curve will be used to determine the concentration of the unknown sample based on its absorbance.
06
Measure the absorbance of the unknown sample using the spectrophotometer. Make sure to properly prepare the sample and handle it with care to avoid any contamination or interference.
07
Use the calibration curve to determine the concentration of the unknown sample based on its absorbance. This can be done by locating the absorbance value on the graph and finding the corresponding concentration.
08
Record the concentration of the unknown sample and any other relevant observations or data obtained during the experiment.
Who needs experiment 3 spectrophotometric determination:
01
Students studying chemistry or related fields who are learning about spectrophotometry and its applications in quantitative analysis.
02
Researchers or scientists who need to determine the concentration of certain compounds or analyze the properties of substances using spectrophotometry.
03
Industries or laboratories that perform quality control or testing of samples, such as pharmaceutical companies, environmental agencies, and food and beverage manufacturers. Spectrophotometric determination can be used to ensure the accuracy and consistency of products or to monitor specific substances in the environment.
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What is experiment 3 spectrophotometric determination?
Experiment 3 spectrophotometric determination is a laboratory procedure used to measure the concentration of a substance in a solution by analyzing the amount of light absorbed or transmitted.
Who is required to file experiment 3 spectrophotometric determination?
Researchers, scientists, or students conducting experiments that involve spectrophotometric determination are required to file experiment 3.
How to fill out experiment 3 spectrophotometric determination?
Experiment 3 spectrophotometric determination is filled out by recording the absorbance readings at specific wavelengths, calculating the concentration of the sample using a standard curve, and reporting the results accurately.
What is the purpose of experiment 3 spectrophotometric determination?
The purpose of experiment 3 spectrophotometric determination is to quantify the concentration of a substance in a solution, determine the purity of a compound, or analyze chemical reactions.
What information must be reported on experiment 3 spectrophotometric determination?
The experiment 3 spectrophotometric determination report must include the sample name, concentration, absorbance readings, calculations used to determine concentration, and any relevant experimental conditions.
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