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From time to time our fish may become sick in order for us to heal them we need to know what the problem is very often the sickness is related to the conditions under which they are being held especially water quality and most especially ammonia levels in the water we've been through water quality we know how to check the water but let's assume it's not a water quality issue let's assume it's a disease issue the first step is to identify accurately which disease organism it is that we are dealing with as the treatments are different for the different species the first step therefore is to prepare the microscope we prepare the microscope by first removing the dust cover that protects it when it is not in use then we remove the lens caps of the lenses and place them somewhere safe where they are ready for use the microscope is now ready for use the next thing we need to do is collect the sample we take the fish in the one hand and a microscope cover slide in the other move the end of the cover slide cross the fish's body several times to get some of the fish's slime onto the microscope cover slide we then place the cover slide on top of a microscope slide in such a way that the mucus is between the two slides, and we squash it down fairly flat next we place the slide onto the stage of the microscope in order to focus we use the fine button or the course button on the microscope in order to move the sample around in fact to move the entire stage moving the stage either up and down or left and right in this particular instance that the back right-hand side is the on/off switch and when you switch it on you can see the light shines from below through the specimen and into the microscope we look down from above with both eyes open and focus the slide to look for disease organisms what you are looking for is movement keep moving the slide specimen up and down and left and right in a regular pattern until you find some sort of disease organism if you find something identify it using them to disease textbooks when we are finished working with a microscope it is important to again pack it away and look after this very important but fragile piece of machinery the first thing we do is switch the light off and remove the microscope slide we then replace the cover lenses and replace the dust cover to keep the microscopes safe next we separate the cover slide from the microscope slide turn the cover slide upside down and clean both of them with a bit of tissue paper you may need to add a little of water to remove any scales that were stuck to the slides when you are looking under the microscope for disease organisms essentially you are looking for movement in this slide you can see small tetrahymena organisms that are moving around rapidly on the slide the background is in fact the scale of a fish a single individual scale which gives you an idea of how many times this has been magnified white spot is another very common organism that you will see on fish it...
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