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Office of ResearchCO2 EUTHANASIA OF RODENTSOffice of Responsible Research Practices Institutional Animal Care and Use CommitteeOverview/Purpose Euthanasia techniques should result in rapid loss of

How to fill out co2 euthanasia of rodents

How to fill out co2 euthanasia of rodents:

1. First, ensure that you have the necessary equipment and materials such as a CO2 chamber or an appropriate euthanasia system, a supply of CO2 gas, and a measuring device to monitor the concentration of CO2 in the chamber during the procedure.
2. Prepare the CO2 chamber by setting it up in a quiet and well-ventilated area to minimize any potential stress to the rodents.
3. Prioritize the safety of yourself and others, wearing appropriate personal protective equipment such as gloves and a lab coat.
4. Carefully place the rodent(s) into the CO2 chamber, ensuring that they have sufficient space to move around comfortably.
5. Gradually introduce the CO2 into the chamber, following the manufacturer's guidelines or protocols. Monitor the concentration of CO2 to ensure that it reaches the recommended level for euthanasia.
6. Maintain the CO2 concentration for a sufficient amount of time to ensure the humane and effective euthanasia of the rodents.
7. After the recommended time, remove the rodents from the CO2 chamber and confirm that they have been euthanized by checking for signs such as lack of respiration and absence of a heartbeat.
8. Dispose of the rodents' bodies according to ethical and legal guidelines, such as by using an approved method of disposal.

Who needs CO2 euthanasia of rodents?

1. Researchers or scientists conducting studies that involve animal models often need to euthanize rodents, including mice and rats, for various reasons such as the completion of experiments, the end of a study, or the necessity to limit population sizes.
2. Veterinary professionals may also need to use CO2 euthanasia when faced with medical issues or terminal illnesses in rodents that cannot be effectively treated or managed.
3. Animal care and welfare organizations sometimes employ CO2 euthanasia to prevent the suffering of rodents that are injured, sick, or deemed unadoptable.

Please note that individual guidelines, local regulations, and ethical considerations may vary, so it is essential to consult relevant authorities or experts in your specific field or location for specific instructions and guidance on the appropriate use of CO2 euthanasia.

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