Get the free Immunoassay for H pylori in fecal specimens

US005871942A Bulleted States Patent 19 11 Patent Number: Lanka et al. 45 54 Date of Patent: 5,871,942 *Feb. 16, 1999 5,610,060 3/1997 Ward .................................. .. 435/252.1 SPECIMENS

How to fill out immunoassay for h pylori

How to fill out immunoassay for H. pylori:

1. Ensure you have the immunoassay kit for H. pylori, which typically includes all the necessary components such as antigen or antibody conjugates, controls, buffers, and a microplate.
2. Begin by labeling the microplate wells accordingly, following the instructions provided in the kit. Typically, the wells are labeled with A, B, C, and so on.
3. Prepare the samples or specimens for testing. This may involve collecting gastric biopsies, serum, or urine samples depending on the type of immunoassay being used.
4. Dilute the samples appropriately as per the instructions provided in the kit. This step is crucial to ensure accurate test results.
5. Add the diluted samples to the designated wells on the microplate. It is important to pipette the correct volume of each sample into its respective well.
6. Incubate the microplate at the recommended temperature and duration specified in the kit instructions. This step allows for the interaction between the H. pylori antigen or antibody and the specific reagents in the kit.
7. After the incubation period, carefully wash the wells to remove any unbound materials or debris. Proper washing techniques should be followed to minimize cross-contamination and ensure accurate results.
8. Add the appropriate conjugate or secondary antibody to each well and incubate it according to the kit instructions. This step facilitates the detection of any bound antigens or antibodies.
9. After the secondary incubation, perform another round of washing to remove any excess conjugate or secondary antibody.
10. Add the substrate or chromogen solution to each well. This will initiate a color change reaction, indicating the presence of H. pylori antigens or antibodies.
11. Allow the appropriate incubation time for the color reaction to develop, as specified in the kit instructions.
12. Finally, stop the color reaction by adding the stop solution provided in the kit.
13. Measure the optical density or absorbance of each well using a microplate reader. Compare the results to the provided cutoff values or reference standards to determine the presence or absence of H. pylori.
14. Record and interpret the results according to the guidelines provided in the kit instructions.

Who needs immunoassay for H. pylori?

1. Individuals experiencing symptoms suggestive of an H. pylori infection, such as persistent abdominal pain, bloating, nausea, vomiting, or unexplained weight loss.
2. Individuals who have a documented history of peptic ulcers, gastric cancer, or gastritis, as H. pylori infection is a common underlying cause for these conditions.
3. Individuals who have completed a course of H. pylori treatment to confirm eradication of the bacteria.
4. Individuals with a family history of H. pylori infection or related gastric diseases.
5. Individuals who require follow-up testing after an initial positive result to monitor treatment response or recurrence.

It is important to consult with a healthcare professional for proper evaluation, diagnosis, and interpretation of immunoassay results for H. pylori.

For pdfFiller’s FAQs

What is immunoassay for h pylori?

Immunoassay for H. pylori is a test that detects antibodies specific to the bacteria Helicobacter pylori in the blood.

Who is required to file immunoassay for h pylori?

Individuals who are suspected to have H. pylori infection or those who have been prescribed treatment for it may be required to undergo immunoassay.

How to fill out immunoassay for h pylori?

Immunoassay for H. pylori usually involves a blood sample collected from the individual, which is then tested for the presence of antibodies to the bacteria.

What is the purpose of immunoassay for h pylori?

The purpose of immunoassay for H. pylori is to diagnose an active infection or to confirm the effectiveness of treatment for the infection.

What information must be reported on immunoassay for h pylori?

The immunoassay for H. pylori report usually includes details such as the patient's name, date of birth, date of the test, test results, and any relevant medical history.

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