Get the free PILOT-SCALE PRODUCTION OF LACTOBACILLUS RHAMNOSUS ATCC 7469 - psasir upm edu

PILOTSCALE PRODUCTION OF LACTOBACILLUS RHAMNOSUS ATC 7469 By LIES VIEW LING Thesis submitted to the School of Graduate Studies, University Petra Malaysia, in Fulfillment of the Requirements for the

How to fill out pilot-scale production of lactobacillus

1. Firstly, gather the necessary equipment and materials for the pilot-scale production of lactobacillus. This may include fermenters, incubators, culture media, and sterilization equipment.
2. Prepare the culture media by combining the required ingredients and sterilize it to ensure a sterile environment for the growth of lactobacillus. This is usually done by autoclaving at high temperature and pressure.
3. Inoculate the lactobacillus culture into the sterilized culture media. This can be done by adding a small amount of the lactobacillus culture to the media and ensuring proper mixing and dispersion.
4. Transfer the inoculated culture media to the fermenters and set the desired temperature, pH, and aeration conditions. Lactobacillus is typically grown under specific conditions to stimulate optimal growth and production.
5. Monitor the growth of lactobacillus regularly by measuring parameters such as cell density, pH, and metabolite production. This helps to ensure that the culture is thriving and producing the desired lactobacillus biomass.
6. Harvest the lactobacillus biomass once it reaches the desired level of growth and production. This can be done by separating the cells from the liquid media using methods like centrifugation or filtration.
7. Store the harvested lactobacillus biomass appropriately to maintain its viability and quality. This may involve freezing the biomass or utilizing lyophilization techniques for long-term storage.

Who needs pilot-scale production of lactobacillus?

1. Pharmaceutical companies: Lactobacillus is commonly used in the production of various pharmaceutical products, such as probiotics and vaccines. Pilot-scale production allows these companies to test and optimize their production processes before scaling up to larger production volumes.
2. Food and beverage industry: Lactobacillus is widely used in the production of fermented foods and beverages, including yogurt, cheese, sauerkraut, and sourdough bread. Pilot-scale production helps companies in this industry to develop and refine their production methods to ensure consistent quality and flavor profiles.
3. Research institutions and academia: Scientists and researchers often require lactobacillus for their studies and experiments. Pilot-scale production enables them to obtain sufficient quantities of lactobacillus for their research purposes, allowing them to advance their understanding of this microorganism and its potential applications.
4. Biotechnology companies: Lactobacillus has diverse applications in the field of biotechnology, including biofuel production, waste treatment, and bioremediation. Pilot-scale production is essential for these companies to develop and optimize their bioprocesses using lactobacillus.

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