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Next era DNA Library Prep Reference Guider Research Use Only. Not for use in diagnostic procedures. ILLUMINE PROPRIETARY Document # 15027987 v01 January 2016Customize a short ended workflow guide
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How to fill out nextera dna library prep
How to fill out nextera dna library prep:
01
Start by preparing your sample DNA. This can be done by isolating the DNA from your desired sample using appropriate methods and protocols.
02
Once your DNA is ready, quantitate the DNA concentration accurately using a fluorometric method or spectrophotometry.
03
Dilute the DNA to the desired concentration according to the instructions provided with the nextera dna library prep kit.
04
Now, prepare the tagmentation reaction mix by combining the appropriate volumes of Tagment DNA Enzyme and Nextera Tagmentation buffer. Follow the manufacturer's instructions for the exact volumes and ratios.
05
Add the diluted DNA to the tagmentation reaction mix and mix gently by pipetting up and down. Ensure thorough mixing of the DNA and the reaction mix.
06
Incubate the tagmentation reaction mix at the recommended temperature for the specified duration. This step allows the Tagment DNA Enzyme to fragment and tag the DNA simultaneously.
07
After the incubation, stop the tagmentation reaction by adding the Neutralize Tagment Buffer. Mix gently to ensure proper neutralization.
08
Clean up the tagmented DNA using appropriate purification methods, such as magnetic bead-based purification or column-based purification. Follow the manufacturer's instructions for the specific method.
09
Once the DNA is purified, prepare the PCR master mix by combining the required volumes of library PCR primer 1, library PCR primer 2, and PCR Master Mix Solution. Check the kit instructions for the exact volumes and ratios.
10
Add the purified tagmented DNA to the PCR master mix and mix gently by pipetting up and down.
11
Run the PCR amplification using appropriate cycling conditions, as provided by the manufacturer. This allows for the amplification of your tagmented DNA fragments.
12
After the PCR amplification, clean up the PCR products using purification methods, such as magnetic bead-based purification or column-based purification. Follow the manufacturer's instructions for the specific method.
13
Finally, quantitate the concentration of your DNA library using fluorometric or spectrophotometric methods. Adjust the library concentration if necessary for downstream applications.
Who needs nextera dna library prep:
01
Researchers or scientists working in the field of genomics who require efficient and streamlined library preparation for DNA sequencing.
02
Those aiming for high-throughput and fast DNA library preparation, as nextera dna library prep offers a rapid and cost-effective solution compared to traditional library preparation methods.
03
Individuals or facilities conducting whole-genome sequencing, targeted sequencing, or other sequencing applications that involve fragmenting and barcoding DNA samples prior to sequencing.
04
Nextera dna library prep is also suitable for researchers who need to prepare libraries from limited amounts of DNA, as it offers low input requirements.
05
Any life science professional or laboratory looking to streamline their library preparation workflow and improve sequencing efficiency may benefit from using nextera dna library prep.
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What is nextera dna library prep?
Nextera DNA library prep is a method used to prepare DNA samples for sequencing by adding adapters to the DNA fragments.
Who is required to file nextera dna library prep?
Researchers or laboratories conducting DNA sequencing experiments may be required to perform Nextera DNA library prep.
How to fill out nextera dna library prep?
To fill out Nextera DNA library prep, one must follow the protocol provided by the manufacturer of the kit used for the preparation.
What is the purpose of nextera dna library prep?
The purpose of Nextera DNA library prep is to prepare DNA samples for sequencing by adding adapters for efficient binding to the sequencing platform.
What information must be reported on nextera dna library prep?
On Nextera DNA library prep, information such as sample ID, DNA concentration, and quality metrics must be reported.
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