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Name: Date: Period: Gram Staining to Identify Prokaryotes Introduction: The gram stain is one of several laboratory procedures which can be used to narrow down the identities of unknown bacteria.
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How to fill out gram staining to identify
How to fill out gram staining to identify:
01
Prepare a heat-fixed slide by placing a few drops of the bacterial culture on a clean glass slide.
02
Allow the slide to air dry, and then pass it through the flame of a Bunsen burner a few times to fix the bacteria onto the slide.
03
Flood the slide with crystal violet stain and let it sit for one minute.
04
Rinse the slide gently with water to remove excess stain.
05
Next, flood the slide with iodine solution and let it sit for one minute. This forms a crystal violet-iodine complex within the bacteria.
06
Rinse the slide gently with water again.
07
Decolorize the slide by adding a few drops of alcohol or acetone, and tilt the slide to allow the decolorizing agent to run down the slide. Stop decolorizing as soon as the purple color stops flowing off the slide.
08
Rinse the slide gently with water to remove the decolorizing agent.
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Finally, counterstain the slide by flooding it with safranin stain and letting it sit for one minute.
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Rinse the slide gently with water once more, and then blot it dry with a paper towel.
Who needs gram staining to identify?
01
Microbiologists: Gram staining is an essential technique used by microbiologists to help identify and classify bacteria based on their cell wall characteristics. It is a fundamental procedure in bacteriology and is used to differentiate bacteria into two major groups, Gram-positive and Gram-negative.
02
Medical professionals: Gram staining plays a crucial role in clinical microbiology and medical diagnostics. It aids in determining the type of bacteria causing an infection, which helps guide appropriate treatment options.
03
Research scientists: Gram staining is commonly used in research laboratories to study bacteria and their interaction with other organisms, as well as to investigate the effects of antimicrobial agents on bacterial cells.
Overall, gram staining is a valuable technique that enables identification and classification of bacteria, making it indispensable for microbiologists, medical professionals, and research scientists.
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What is gram staining to identify?
Gram staining is a technique used to differentiate between bacteria based on their cell wall composition.
Who is required to file gram staining to identify?
Laboratory technicians or microbiologists typically perform gram staining to identify bacteria.
How to fill out gram staining to identify?
Gram staining involves staining bacterial cells with crystal violet, iodine, alcohol, and safranin in specific steps.
What is the purpose of gram staining to identify?
The purpose of gram staining is to categorize bacteria into Gram-positive or Gram-negative, aiding in identification and treatment.
What information must be reported on gram staining to identify?
The results of gram staining, including the color of the bacteria after staining and their cellular morphology, must be reported.
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