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Antibody Human Angiogenesis Array 3 Quantitative measurement of 30 human cytokines Patent Pending Technology User Manual (Version July 2010) Cat # QAHANG3 Biotech, Inc. We Provide You With Excellent
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How to fill out quantibody human angiogenesis array
How to fill out quantibody human angiogenesis array:
01
Make sure you have all the necessary equipment and reagents ready before beginning the array filling process.
02
Thaw the quantibody array slide at room temperature for 20 minutes.
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Prepare the blocking buffer and add it to the quantibody array slide, ensuring that the entire surface is covered.
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Incubate the slide in a humid container at room temperature for 30 minutes to block any non-specific binding sites.
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While the slide is incubating, dilute the samples or standards according to the provided instructions and prepare the necessary detection antibodies.
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After the blocking step, carefully remove the blocking buffer and add the diluted samples or standards to the quantibody array slide.
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Incubate the slide overnight at 4°C to allow the proteins in the samples to bind to their respective capture antibodies on the slide.
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The next day, wash the slide multiple times with the provided wash buffer to remove any unbound proteins.
09
Add the prepared detection antibodies to the slide and incubate at room temperature for 2 hours to allow the detection antibodies to bind to the captured proteins.
10
Wash the slide again to remove any unbound detection antibodies.
11
Add the provided fluorophore-conjugated streptavidin solution to the slide and incubate for 30 minutes to allow the streptavidin to bind to the detection antibodies.
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Finally, wash the slide to remove any unbound streptavidin and allow it to air dry.
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Once dried, the quantibody array slide is ready to be scanned and analyzed using the appropriate imaging system and software provided by the manufacturer.
Who needs quantibody human angiogenesis array?
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Researchers studying vascular biology and angiogenesis mechanisms in humans may find the quantibody human angiogenesis array useful.
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Pharmaceutical companies developing drugs targeting angiogenesis pathways can benefit from using the quantibody human angiogenesis array to assess the efficacy and safety of their compounds.
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Clinicians and medical professionals investigating angiogenic biomarkers for diagnostic or prognostic purposes may also utilize the quantibody human angiogenesis array.
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What is quantibody human angiogenesis array?
The quantibody human angiogenesis array is a high-throughput multiplex ELISA microarray used to study angiogenesis-related proteins in human samples.
Who is required to file quantibody human angiogenesis array?
Researchers, scientists, or institutions conducting studies on angiogenesis in humans may be required to file the quantibody human angiogenesis array.
How to fill out quantibody human angiogenesis array?
The quantibody human angiogenesis array is typically filled out by adding samples to the microarray wells, incubating, washing, and then detecting the levels of angiogenesis-related proteins.
What is the purpose of quantibody human angiogenesis array?
The purpose of the quantibody human angiogenesis array is to analyze and quantify the levels of angiogenesis-related proteins in human samples for research or diagnostic purposes.
What information must be reported on quantibody human angiogenesis array?
The quantibody human angiogenesis array typically reports the levels of various angiogenesis-related proteins in the samples being studied.
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