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This manual provides guidelines for the use of the Quantibody® Mouse Cytokine Antibody Array 3000, a product used to quantitatively measure 160 mouse cytokines through a multiplexed sandwich ELISA-based
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How to fill out quantibody mouse cytokine antibody
How to fill out Quantibody® Mouse Cytokine Antibody Array 3000
01
Prepare your samples: Collect and centrifuge supernatants from cell cultures or tissue extracts.
02
Dilute the samples: Depending on the protocol, dilute your samples to the appropriate concentration as indicated in the instruction manual.
03
Prepare the array: Place the Quantibody® Mouse Cytokine Antibody Array 3000 in a clean, flat area.
04
Add samples: Carefully pipette the diluted samples onto the corresponding spots on the array.
05
Incubate: Cover the array with a lid or seal and incubate for the recommended time at room temperature or at 4°C as per the protocol.
06
Wash the array: After incubation, wash the array with the provided wash buffer to remove unbound proteins.
07
Add detection antibodies: Pipette the detection antibodies to the array and incubate again as per instructions.
08
Final wash: Wash the array thoroughly to remove excess detection antibodies.
09
Substrate addition: Add substrate solution to visualize the detected proteins on the array.
10
Analyze results: Use imaging equipment to capture and quantify the results.
Who needs Quantibody® Mouse Cytokine Antibody Array 3000?
01
Researchers studying cytokine profiles in mouse models.
02
Laboratories conducting immunology or inflammation research.
03
Pharmaceutical companies developing therapeutics targeting cytokines.
04
Academic institutions focusing on cytokine signaling and immune responses.
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What is Quantibody® Mouse Cytokine Antibody Array 3000?
Quantibody® Mouse Cytokine Antibody Array 3000 is a multiplex assay designed to quantify various cytokines in mouse samples simultaneously, enabling researchers to examine immune responses and inflammatory processes.
Who is required to file Quantibody® Mouse Cytokine Antibody Array 3000?
Researchers and laboratories conducting studies on cytokine levels in mouse models are typically required to file the Quantibody® Mouse Cytokine Antibody Array 3000 for accurate assay results and data documentation.
How to fill out Quantibody® Mouse Cytokine Antibody Array 3000?
To fill out the Quantibody® Mouse Cytokine Antibody Array 3000, users should follow the provided protocol which includes sample preparation, dilution, and application of the samples to the assay plates, ensuring all steps are conducted under recommended conditions.
What is the purpose of Quantibody® Mouse Cytokine Antibody Array 3000?
The purpose of the Quantibody® Mouse Cytokine Antibody Array 3000 is to enable detailed profiling of cytokine expression in mouse models, aiding in the understanding of disease mechanisms and evaluation of therapeutic responses.
What information must be reported on Quantibody® Mouse Cytokine Antibody Array 3000?
The information that must be reported includes the types and concentrations of cytokines detected, assay controls, sample details, and any relevant experimental conditions that could impact the results.
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