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Quantitative measurement of 20 Human TH1-TH2-TH17 cytokines with detailed instructions on usage, protocols, and troubleshooting.
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How to fill out quantibody human th17 array
How to fill out Quantibody® Human TH17 Array 1
01
Gather all necessary materials including the Quantibody® Human TH17 Array 1 kit and a microplate reader.
02
Prepare samples by diluting them to the recommended concentrations specified in the protocol.
03
Set up the assay by adding the diluted samples to the designated wells of the array plate.
04
Incubate the plate at the specified temperature and duration as mentioned in the instructions.
05
Wash the plate to remove unbound substances using the recommended wash buffer.
06
Add detection antibodies to each well according to the protocol.
07
Incubate again to allow binding of detection antibodies to the target analytes.
08
Perform washing steps to eliminate excess detection antibodies.
09
Add substrate solution to the wells to initiate the signal reaction.
10
Stop the reaction after the appropriate time and read the results using a microplate reader.
Who needs Quantibody® Human TH17 Array 1?
01
Researchers studying TH17 cells and related immune responses.
02
Clinical laboratories assessing inflammatory conditions and autoimmune diseases.
03
Pharmaceutical companies developing therapies targeting TH17 pathway.
04
Academic institutions conducting immunology research.
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What is Quantibody® Human TH17 Array 1?
Quantibody® Human TH17 Array 1 is a specialized multiplex assay designed to simultaneously measure the levels of various cytokines associated with TH17 cell activity in human samples.
Who is required to file Quantibody® Human TH17 Array 1?
Researchers and laboratories conducting studies on TH17 cells and related immune responses are required to use and file Quantibody® Human TH17 Array 1 for their experimental data and findings.
How to fill out Quantibody® Human TH17 Array 1?
To fill out the Quantibody® Human TH17 Array 1, users must follow specific protocols outlined in the product manual, including sample preparation, incubation times, and data analysis procedures.
What is the purpose of Quantibody® Human TH17 Array 1?
The purpose of Quantibody® Human TH17 Array 1 is to facilitate the understanding of TH17 cell-mediated immune responses and their implications in various diseases by providing comprehensive cytokine profiles.
What information must be reported on Quantibody® Human TH17 Array 1?
The information that must be reported includes the concentrations of measured cytokines, sample identifiers, experimental conditions, and any relevant control data.
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