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Hello my name is Brendan Brink man and the senior product manager for laser scanning confocal microscopes at OlympusAmerica'’s scientific equipment group today IN#39’m going to show you just how easy confocal imaging can be with the FE10 auto turn the system on all you have to do press this button you#39’ll note that the system already has power from the power supply unit by seeing this red LED indicator light if I press this button and hold until I hear a beep the system will start in Nan incubation mode toucan tell it#39’s in Nan incubation mode by seeing these two dash lines in the LED display windows the first thing we do after we turnon the instrument is decided what kind of sample holder we want to use the FE 10icomes with a range of different kinds of sample holders everything from simple slides to a culture pod which allows youth acquire multiple days of time-lapsedata for today#39’s demonstration IN#39’m just going to use the slide holder notice that the slide holder has five different lanes these correspond to different imaging lanes that you will see represented in cartoon form on the screen the first thing IN#39’m going to does take my slide place is immanent in animating Lane IN#39’ll come to the instrument open the lid and place the sample holder inside the FE 10 I this happens to be Anne 10 I live which allows for long-termtime lapse, so we have this extra culture cover IN#39’ll place that inside the instrument close the lid and now IN#39;ready to begin imaging so the first thing we do decide what dies we want to image with the F feet NI as you can see there area large range of dyes already in the duelist in addition to some preset dyes such as the Alexa floors and also green fluorescent protein and yellow fluorescent protein we have general selections for blue narrow green narrow and so on, so the system is quite flexible in terms of what you want to image for the sample that I have now#39’m going to use the blue narrow green narrow and red narrow selections if Wanted to remove one of these I could select it and delete it or select all clear then come back in double-click on the dies, and they repopulate into the selected dyes window additionally if Wanted to load from an acquired image Could select this tab and then immediately pull up from a previous experiment that way I could easily replicate what I had done before the next step is to click start the FE tonight has two fluorescent PMT#39;for doing the map view we select two colors for the imaging once I click okay we have the grid which represents all the different fields that could be selected for scanning the sample that I placed in the system waste slide holder, and you can see themulti-channel lanes here for the slide holder if I wanted to change which lane was going to MAPI would simply double-click on it, and it would move over to that position since Know IN#39’m in position 3 IN#39’m going to select position 3I could also register multiple containers and have the...
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