Get the free Poietics Human Preadipocyte Cryoplate - Lonza Group

Monza Cartersville, Inc. www.lonza.com scientific. Support lonza.com Scientific Support: 8005210390 Document # IS001909531 12/09 Cartersville, MD 217930127 USA 2009 Monza Cartersville, Inc. Poetics

How to fill out poietics human preadipocyte cryoplate

How to fill out poietics human preadipocyte cryoplate:

1. Start by preparing the cryoplate: Ensure that the cryoplate is clean and free of any contaminants. Use sterile techniques and allow the cryoplate to reach room temperature.
2. Thaw the preadipocytes: Take the vial containing the poietics human preadipocytes and thaw them in a 37°C water bath for a few minutes until completely melted. Gently vortex the vial to ensure the cells are mixed well.
3. Prepare the medium: Meanwhile, prepare the appropriate growth medium or freezing medium according to the instructions provided by the manufacturer. Ensure the medium is at the recommended temperature.
4. Dilute the preadipocytes: Take the thawed preadipocytes and dilute them in the prepared medium to the desired concentration or cell density. This step may require pipetting a specific volume of the preadipocytes and adding it to the medium. Gently mix to ensure uniform distribution.
5. Fill the cryoplate: Carefully dispense the diluted preadipocytes into the wells of the cryoplate. Start from one corner and systematically fill each well, ensuring that the same volume and cell density are maintained throughout.
6. Record the necessary information: It is crucial to label the cryoplate with the necessary information such as the date, cell type, passage number, and any other relevant details. This will help in accurately tracking the cryoplate and its contents.
7. Seal and secure the cryoplate: Once all the wells are filled, seal the cryoplate tightly using a compatible lid or cover. Make sure it is secured to prevent any contamination or evaporation during the freezing process.
8. Transfer to a controlled rate freezer: Place the sealed cryoplate in a controlled rate freezer that can gradually reduce the temperature. Follow the recommended freezing protocol specific to the preadipocytes or as provided by the manufacturer.
9. Store in liquid nitrogen: After the controlled rate freezing, transfer the cryoplate to a storage container or dewar filled with liquid nitrogen. Ensure the cryoplate is stored vertically and can be easily retrieved when needed.

Who needs poietics human preadipocyte cryoplate?

1. Researchers and scientists studying adipose tissue: Preadipocytes are essential cells for studying the biology of adipose tissue, its development, and related diseases such as obesity.
2. Biomedical companies: Companies involved in drug discovery, tissue engineering, or regenerative medicine may require poietics human preadipocyte cryoplates for their research and development projects.
3. Medical professionals and clinicians: Poietics human preadipocyte cryoplates may be needed by medical professionals and clinicians involved in adipose tissue transplantation or surgical procedures requiring adipose-derived cells.

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What is poietics human preadipocyte cryoplate?

Poietics human preadipocyte cryoplate is a form used to document the cryopreservation of preadipocyte cells for research purposes.

Who is required to file poietics human preadipocyte cryoplate?

Researchers or scientists conducting studies involving preadipocyte cells are required to file poietics human preadipocyte cryoplate.

How to fill out poietics human preadipocyte cryoplate?

The form should be completed with details of cryopreservation methods, cell characteristics, and storage conditions according to the provided guidelines.

What is the purpose of poietics human preadipocyte cryoplate?

The purpose of poietics human preadipocyte cryoplate is to ensure proper documentation and tracking of preadipocyte cell cryopreservation for research integrity and reproducibility.

What information must be reported on poietics human preadipocyte cryoplate?

Information such as cell source, cryopreservation protocol, freezing and storage conditions, and quality control measures must be reported on poietics human preadipocyte cryoplate.