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This document provides information on HaloTag® ORF clones, applications of HaloTag® technology, and a form to order HaloTag® ORF clones, including pricing and promotional details.
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How to fill out halotag orf clones
How to fill out HaloTag® ORF Clones
01
Obtain the HaloTag® ORF Clone relevant to your target protein.
02
Prepare a culture of E. coli suitable for transformation.
03
Use a restriction enzyme to linearize the plasmid if required.
04
Transform the E. coli with the HaloTag® ORF Clone DNA.
05
Plate the transformed E. coli on selective agar to isolate colonies.
06
Incubate the plates to allow growth of successful transformants.
07
Screen colonies using PCR or restriction digestion to confirm the presence of the insert.
08
Grow confirmed colonies in liquid culture for plasmid extraction.
09
Purify the plasmid DNA for downstream applications such as transfection.
Who needs HaloTag® ORF Clones?
01
Researchers working in molecular biology who require protein tagging.
02
Laboratories seeking to study protein interactions and localization.
03
Companies focused on drug discovery and development involving target proteins.
04
Academic institutions conducting research on gene expression and function.
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What is HaloTag® ORF Clones?
HaloTag® ORF Clones are a series of genetically engineered constructs designed to facilitate the study of protein function and interactions in live cells. These clones express a HaloTag fusion protein that can be covalently labeled with a variety of chemical probes.
Who is required to file HaloTag® ORF Clones?
Researchers or laboratories that utilize HaloTag® ORF Clones for experiments in molecular biology, genetics, or related fields are typically required to file any necessary documentation related to their use, depending on institutional guidelines or funding requirements.
How to fill out HaloTag® ORF Clones?
To fill out HaloTag® ORF Clones, researchers should provide detailed information such as the specific clone identifier, the target gene or protein, experimental conditions, and any labeling agents used. It is essential to follow any provided guidelines or templates by the issuing authority.
What is the purpose of HaloTag® ORF Clones?
The purpose of HaloTag® ORF Clones is to allow scientists to tag and visualize proteins of interest in living cells, enabling studies on protein expression, localization, and interaction dynamics in real-time.
What information must be reported on HaloTag® ORF Clones?
Information that must be reported includes details such as the clone name, vector information, gene of interest, experimental methods, results, and any safety or compliance information relevant to the use of the clones.
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