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AntiRibosomal P (Egg) ELISA For the quantitative and qualitative detection of antibodies against ribosomal Proteins (ribs) in serum. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog
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How to fill out anti-ribosomal p igg elisa
How to Fill Out Anti-Ribosomal P IgG ELISA:
01
Prepare the samples: Collect the serum samples from the individuals who need to be tested for the presence of anti-ribosomal P IgG. Properly label each sample and ensure they are properly stored and refrigerated.
02
Dilute the samples: Dilute the serum samples with the provided diluent solution according to the instructions provided with the ELISA kit. This step helps in achieving an optimal concentration for accurate results.
03
Prepare the ELISA plate: Take the ELISA plate and label each well according to the sample being tested. Add the diluted samples to the appropriate wells using a multichannel pipette or a pipette as instructed.
04
Incubation: Allow the plate to incubate for the specified time at the recommended temperature to allow the anti-ribosomal P IgG antibodies present in the samples to bind to the specific antigen on the plate.
05
Wash the plate: After the incubation, wash the plate thoroughly to remove any unbound substances or contaminants. Follow the recommended wash steps provided with the ELISA kit.
06
Add the detection reagent: Add the detection reagent, which typically consists of an enzyme conjugated to an anti-human IgG antibody, to each well. This reagent will bind to any bound anti-ribosomal P IgG, forming a detectable complex.
07
Incubation and wash: Allow the plate to incubate for the specified time at the recommended temperature to facilitate the binding of the detection reagent. Then, wash the plate thoroughly to remove any unbound detection reagent.
08
Add substrate: Add the substrate solution to the wells, which will react with the enzyme linked to the detection reagent. This reaction leads to the development of a color change, typically from colorless to blue or yellow.
09
Stop the reaction: After a suitable incubation time, add the stop solution to halt the enzymatic reaction. This will change the color of the solution from blue or yellow to a stable color, usually green or orange, indicating the presence or absence of anti-ribosomal P IgG.
10
Read the results: Use a microplate reader to measure the absorbance of each well at the appropriate wavelength specified in the kit's instructions. Compare the results to the provided control values or reference ranges to determine the presence and quantity of anti-ribosomal P IgG in each sample.
Who Needs Anti-Ribosomal P IgG ELISA:
Anti-ribosomal P IgG ELISA is typically used in the field of autoimmune diagnostics. It is specifically used to detect and quantify the presence of anti-ribosomal P IgG antibodies in serum samples. This antibody is associated with certain autoimmune diseases, such as systemic lupus erythematosus (SLE) and central nervous system (CNS) lupus. Therefore, individuals suspected of having these conditions or related autoimmune disorders may require an anti-ribosomal P IgG ELISA test to aid in diagnosis and monitoring of the disease.
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What is anti-ribosomal p igg elisa?
Anti-ribosomal P IgG ELISA is a test used to detect antibodies against ribosomal P proteins in the blood, which can be a marker for certain autoimmune diseases.
Who is required to file anti-ribosomal p igg elisa?
Patients who have been prescribed the test by their healthcare provider are required to take the test and file the results.
How to fill out anti-ribosomal p igg elisa?
The test is typically conducted by a healthcare provider, who will take a blood sample from the patient and send it to a lab for analysis.
What is the purpose of anti-ribosomal p igg elisa?
The purpose of the test is to diagnose autoimmune diseases that may be associated with the presence of antibodies against ribosomal P proteins.
What information must be reported on anti-ribosomal p igg elisa?
The test results, including the levels of antibodies detected, must be reported on the anti-ribosomal P IgG ELISA test.
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