Get the free Anti-M2 IgG ELISA - ALPCO

AntiM2 (Egg) ELISA For the quantitative and qualitative detection of antibodies against M2 in serum. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: Size: Version: 35M2GHUE01

How to fill out anti-m2 igg elisa

How to fill out anti-m2 igg elisa:

1. Obtain the necessary materials and reagents for the anti-m2 igg elisa assay, including the microplates, antibodies, antigens, wash buffers, and enzyme substrate.
2. Thaw and mix all components thoroughly according to the manufacturer's instructions.
3. Prepare the samples by diluting the patient's serum in the appropriate dilution buffer.
4. Dispense the diluted samples and controls into the appropriate wells of the microplate, following the plate map provided.
5. Incubate the plate at the specified temperature for the recommended duration to allow antibody-antigen binding to occur.
6. Wash the microplate wells with the wash buffer to remove any unbound materials.
7. Add the anti-human igg enzyme conjugate to each well and incubate again to allow for the formation of immune complexes.
8. Wash the microplate wells again to remove any unbound conjugate.
9. Add the enzyme substrate, typically a chromogenic substrate, to each well, and incubate to allow the enzymatic reaction to occur.
10. Finally, measure the absorbance of each well using a microplate reader and record the results.

Who needs anti-m2 igg elisa?

1. Patients suspected of having primary biliary cholangitis (PBC), as anti-m2 igg antibodies are a specific biomarker for this autoimmune liver disease.
2. Individuals with symptoms such as fatigue, itching, jaundice, or abdominal pain, which are commonly associated with PBC.
3. Healthcare professionals who require a reliable diagnostic tool to identify and monitor the progression of PBC in their patients.

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What is anti-m2 igg elisa?

Anti-M2 IgG ELISA is a laboratory test used to detect antibodies against M2 subtype of muscarinic acetylcholine receptor.

Who is required to file anti-m2 igg elisa?

Patients with suspected autoimmune liver diseases may be required to undergo anti-M2 IgG ELISA testing.

How to fill out anti-m2 igg elisa?

Anti-M2 IgG ELISA test should be conducted by trained healthcare professionals following the recommended protocols.

What is the purpose of anti-m2 igg elisa?

The purpose of anti-M2 IgG ELISA is to aid in the diagnosis of certain autoimmune liver diseases.

What information must be reported on anti-m2 igg elisa?

The results of the anti-M2 IgG ELISA test, along with patient details and any relevant medical history, must be reported.