Get the free Real Time PCR Service Request Form - University of Hong Kong - cgs hku

Real Time PCR Service Request Form Website: CGS.HK.HK Contact Person: Change Wu / KW Wong / Anita Wong / Clement Chow / Carlos Choir Email: RPC. CGS HK.HK Contact No.: 28315467 × 28315477 Order No.:

How to fill out real time pcr service

How to fill out real-time PCR service?

1. Begin by preparing the necessary reagents and samples for the real-time PCR service. This includes the DNA or RNA samples to be tested, as well as the master mix and primers specific to the target genes.
2. Label the PCR tubes or plates accordingly to ensure proper identification throughout the process.
3. Pipette the master mix into the designated wells or tubes. The master mix typically contains the necessary components for PCR amplification, such as Taq polymerase, dNTPs, buffer, and fluorescent dyes.
4. Add the DNA or RNA samples to the appropriate wells or tubes, ensuring accurate volume measurements.
5. Carefully seal the tubes or plates to prevent contamination and maintain sample integrity.
6. Set up the real-time PCR instrument according to the manufacturer's instructions. This may involve inputting necessary parameters such as the cycling conditions, annealing temperatures, and extension times.
7. Place the sealed tubes or plates into the real-time PCR instrument and start the run. The instrument will perform the cycling process, which includes denaturation, annealing, and extension steps.
8. Monitor the real-time PCR amplification in the instrument's software interface. This will allow you to visualize the amplification curves and measure the fluorescence intensity in each well or tube.
9. Analyze the real-time PCR data using appropriate software. This may involve determining the cycle threshold (Ct) values, calculating relative gene expression levels, or identifying genetic mutations.

Who needs real-time PCR service?

1. Researchers in molecular biology and genetics who need to detect and quantify gene expression levels in specific samples.
2. Pharmaceutical companies conducting drug development research and needing to validate drug efficacy or study drug-target interactions.
3. Clinical laboratories and healthcare professionals using real-time PCR for diagnostic purposes, such as detecting infectious agents, genetic disorders, or identifying cancer mutations.
4. Forensic scientists utilizing real-time PCR to analyze DNA evidence in criminal investigations or paternity testing.
5. Environmental scientists studying microbial ecology, water quality, or agricultural biotechnology often rely on real-time PCR for accurate identification and quantification of target organisms.

In summary, real-time PCR services are utilized by a wide range of professionals and industries for various applications, including research, diagnostics, drug development, forensics, and environmental studies. The process of filling out a real-time PCR service involves preparing reagents and samples, pipetting the master mix and samples, setting up the instrument, running the amplification, monitoring the data in real-time, and analyzing the results.

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What is real time pcr service?

Real time PCR service is a technique used for detecting the presence of a specific DNA sequence in real time.

Who is required to file real time pcr service?

Researchers, laboratories, or institutions conducting genetic testing or research may be required to file real time PCR service.

How to fill out real time pcr service?

To fill out real time PCR service, one needs to provide information about the DNA sequence being tested, the samples being used, and the results of the test.

What is the purpose of real time pcr service?

The purpose of real time PCR service is to accurately and quickly detect the presence of a specific DNA sequence in a sample.

What information must be reported on real time pcr service?

Information such as the DNA sequence being tested, the samples used, the testing procedure, and the results obtained must be reported on real time PCR service.