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Material Safety Data Sheet YMO Research advises each customer or recipient of this MSDS to study it carefully to become aware of and understand the hazards associated with the product. The reader

How to fill out his-spin nickel affinity gel

How to fill out His-Spin Nickel Affinity Gel:

1. First, prepare the His-Spin Nickel Affinity Gel by equilibrating it with an appropriate buffer solution. Follow the manufacturer's instructions for the specific gel you are using.
2. Take the prepared His-Spin Nickel Affinity Gel and gently mix it to ensure the nickel ions are uniformly distributed throughout the gel matrix.
3. Next, carefully load the His-Spin Nickel Affinity Gel into a column or cartridge. Make sure to use a column or cartridge that is recommended for the specific gel you are using.
4. Before applying your sample, wash the His-Spin Nickel Affinity Gel with the equilibration buffer or a compatible wash buffer. This helps remove any impurities and prepares the gel for efficient binding of the target protein.
5. After washing, apply your sample containing the target protein onto the His-Spin Nickel Affinity Gel. Allow sufficient contact time for the protein to bind to the nickel ions in the gel matrix. This binding is based on the affinity of the histidine-tagged protein for nickel.
6. Once the binding is complete, wash the gel with an appropriate wash buffer to remove any unbound or nonspecifically bound molecules. The number and type of washes will depend on the specific protocol or manufacturer's instructions.
7. Finally, elute or recover the specifically bound protein from the His-Spin Nickel Affinity Gel using an elution buffer. The elution buffer disrupts the interaction between the nickel ions and the histidine tag, releasing the target protein.

Who needs His-Spin Nickel Affinity Gel:

1. His-Spin Nickel Affinity Gel is commonly used by researchers and scientists involved in protein purification. It is particularly useful for purifying proteins that contain a histidine-tag, also known as His-tag or 6xHis-tag.
2. Researchers working with recombinant proteins expressed in bacterial, yeast, or insect expression systems often employ His-Spin Nickel Affinity Gel for purification purposes. The histidine tag allows for specific binding to the nickel ions present in the gel matrix, enabling efficient isolation of the target protein from a complex mixture.
3. The His-Spin Nickel Affinity Gel is also useful in various applications such as protein-protein interaction studies, affinity chromatography, and antibody purification.

In summary, the His-Spin Nickel Affinity Gel provides a reliable and efficient method for purifying histidine-tagged proteins, making it a valuable tool for protein purification in research and biotechnology laboratories.

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What is his-spin nickel affinity gel?

His-spin nickel affinity gel is a type of chromatography resin used to purify proteins containing a polyhistidine (His-tag) affinity tag.

Who is required to file his-spin nickel affinity gel?

Researchers or scientists working with proteins that have been tagged with a polyhistidine affinity tag may need to use his-spin nickel affinity gel.

How to fill out his-spin nickel affinity gel?

His-spin nickel affinity gel is typically used in a chromatography column setup. The protein sample is loaded onto the column and washed with buffer before eluting the target protein with imidazole-containing elution buffer.

What is the purpose of his-spin nickel affinity gel?

The purpose of his-spin nickel affinity gel is to selectively purify proteins that have been tagged with a polyhistidine affinity tag, allowing for efficient protein purification.

What information must be reported on his-spin nickel affinity gel?

The information reported on his-spin nickel affinity gel would typically include the protein sample, buffer composition, chromatography conditions, elution profile, and protein yield.