Get the free Nile Red A Selective Fluorescent Stain for Intracellular Lipid Droplets - jcb rupress

Published March 1, 1985, Nile Red A Selective Fluorescent Stain for Intracellular Lipid Droplets PHILLIP GREENSPAN, EUGENE P. MAYER, and STANLEY D. FOWLER Department of Pathology and Department of

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How to fill out Nile Red a selective:

1. Prepare a solution: Dissolve Nile Red dye in a suitable solvent, such as dimethyl sulfoxide (DMSO), at the desired concentration. It is important to ensure that the dye is completely dissolved.
2. Select the target cells or structures: Determine the specific cells or structures that you want to stain with Nile Red. This could be lipid droplets, membranes, or other lipid-rich components.
3. Prepare the sample: If working with cells, grow them to the desired confluency and fix them using an appropriate fixative if necessary. For tissues or organisms, prepare thin sections or whole-mount samples.
4. Incubate with Nile Red: Apply the Nile Red solution to the sample for a suitable period of time, typically 10-30 minutes, depending on the sample type and concentration of dye. Make sure the sample is completely covered by the solution.
5. Wash and mount: Remove the excess Nile Red solution by rinsing the sample with an appropriate buffer or solvent. Gently blot or air-dry the sample and then mount it on a glass slide using a mounting medium, such as glycerol or DAPI-containing medium.
6. Visualization: Observe the stained sample using a fluorescence microscope equipped with an appropriate excitation wavelength (around 488-520 nm) and emission filter (around 560-620 nm) for Nile Red. Adjust the microscope settings to optimize the signal-to-noise ratio.
7. Image acquisition and analysis: Capture images of the stained sample using a suitable camera or imaging system. Perform image analysis to quantify or measure the target cells or structures stained with Nile Red.

Who needs Nile Red a selective?

1. Researchers studying lipid metabolism: Nile Red is commonly used to visualize and quantify lipid droplets in cells or tissues. Researchers interested in understanding lipid metabolism, lipid storage, or lipid-related diseases can benefit from Nile Red staining.
2. Microbiologists studying lipophilic bacteria: Some bacteria or microorganisms possess lipophilic characteristics, and Nile Red can be used to stain these lipophilic bacteria for identification or enumeration in environmental or microbiological studies.
3. Pharmacologists studying drug delivery systems: Nile Red has been used to investigate drug delivery systems based on liposomes, nanoparticles, or other lipid-based carriers. Researchers studying drug release, drug interactions with lipid membranes, or drug encapsulation can utilize Nile Red staining in their studies.
4. Environmental scientists studying oil spills or pollutant monitoring: Nile Red has been employed for monitoring and assessing the presence of hydrocarbons or oil spills in environmental samples. Its ability to selectively stain hydrophobic compounds makes it a valuable tool for environmental monitoring and pollution assessment.

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What is nile red a selective?

Nile red is a selective fluorescent stain used in various applications, such as lipid staining and detection of neutral lipids.

Who is required to file nile red a selective?

There is no specific requirement to file nile red a selective as it is a fluorescent stain used in laboratories and research settings.

How to fill out nile red a selective?

Nile red is not something that needs to be filled out. It is a chemical dye used in fluorescence microscopy and various biochemical assays.

What is the purpose of nile red a selective?

The purpose of nile red is to selectively stain and detect neutral lipids, such as triglycerides and cholesterol esters, in various biological samples.

What information must be reported on nile red a selective?

There is no specific information that needs to be reported for nile red as it is a chemical stain used in scientific research.