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Technical specifications for the Mouse Anti-Hsp70 Alkaline Phosphatase Conjugate Monoclonal Antibody, detailing its immunogen, specificity, applications, and usage instructions.
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How to fill out Anti-Hsp70:AP Conjugate
01
Gather all necessary materials: Anti-Hsp70:AP conjugate, diluent, an appropriate microplate or test tube, and pipettes.
02
Determine the required dilution of the Anti-Hsp70:AP conjugate as per the protocol or instructions provided.
03
Measure the appropriate volume of the conjugate using a pipette.
04
Add the measured volume of the Anti-Hsp70:AP conjugate to the diluent in the microplate or test tube.
05
Mix the solution gently to ensure thorough mixing without creating bubbles.
06
Use the diluted Anti-Hsp70:AP conjugate immediately or store it according to the manufacturer's instructions if necessary.
Who needs Anti-Hsp70:AP Conjugate?
01
Researchers studying cellular stress responses and protein interactions.
02
Clinicians involved in diagnosing autoimmune diseases, particularly related to heat shock proteins.
03
Laboratories conducting assays related to protein expression and functionality.
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People Also Ask about
What is the function of Hsp70 DnaK?
Molecular chaperones of the Hsp70 family are conserved proteins that modulate intracellular protein folding. By binding to unfolded or partially folded polypeptides, chaperones prevent misfolding and aggregation and promote folding, translocation, and the assembly and disassembly of multiprotein structures (1, 2).
What is the function of Hsp70?
Hsp70 proteins are central components of the cellular network of molecular chaperones and folding catalysts. They assist a large variety of protein folding processes in the cell by transient association of their substrate binding domain with short hydrophobic peptide segments within their substrate proteins.
What is HSP70 antibody?
HSP70 Antibody detects endogenous levels of total HSP70 protein (HSP70-Hom, HSP70-1) and HSC70. Human, Mouse, Rat, Monkey, Bovine. Goat.
What is the function of the mitochondrial Hsp70?
Members of the 70-kDa heat shock protein (hsp70) family play an essential role in protein folding, transport of proteins into different cellular compartments, and regulation of the heat shock response (1, 2). The members of this protein family are highly conserved from bacteria to man (3, 4).
What is the function of BiP Hsp70?
BiP, an HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER), binds newly-synthesized proteins as they are translocated into the ER and maintains them in a state competent for subsequent folding and oligomerization.
What is the expression of HSP70?
Gene expression patterns of the Hsp70 gene in different organs indicated that the liver, spleen, brain and kidney exhibited 5.94, 4.96, 5.29 and 2.63-fold higher mRNA level expressions than the control (Table 1). Liver and brain tissues showed the highest gene expression levels as compared to kidney, spleen and heart.
What does HSP70 do?
Hsp70 proteins are central components of the cellular network of molecular chaperones and folding catalysts. They assist a large variety of protein folding processes in the cell by transient association of their substrate binding domain with short hydrophobic peptide segments within their substrate proteins.
What is the response of heat shock HSP70?
Heat shock protein 70 (Hsp70) is a molecular chaperone that is expressed in response to stress. In this role, Hsp70 binds to its protein substrates and stabilize them against denaturation or aggregation until conditions improve.
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What is Anti-Hsp70:AP Conjugate?
Anti-Hsp70:AP Conjugate is a bioconjugate formed by linking anti-Hsp70 antibodies with alkaline phosphatase (AP), used in various laboratory assays to detect the presence of Hsp70 proteins.
Who is required to file Anti-Hsp70:AP Conjugate?
Researchers and laboratories that are conducting experiments or assays involving the detection of Hsp70 proteins using Anti-Hsp70:AP Conjugate are typically required to file necessary documentation regarding its use.
How to fill out Anti-Hsp70:AP Conjugate?
To fill out Anti-Hsp70:AP Conjugate, ensure that all relevant details regarding the conjugate's composition, usage, and experimental context are provided, including the concentrations, assay methods, and any controls used.
What is the purpose of Anti-Hsp70:AP Conjugate?
The purpose of Anti-Hsp70:AP Conjugate is to facilitate the detection and quantification of Hsp70 proteins in research and clinical settings, aiding in studies of stress response and cellular function.
What information must be reported on Anti-Hsp70:AP Conjugate?
Information that must be reported includes the source of the antibodies, concentration, storage conditions, lot number, experimental protocols, and any relevant safety information.
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