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This document provides technical specifications, application notes, safety data, and storage information for the Recombinant Bovine Hsc70 Protein-ATPase Fragment product.

How to fill out hsc70 protein-atpase fragment

How to fill out Hsc70 Protein-ATPase Fragment

1. Start by obtaining the Hsc70 Protein-ATPase Fragment and ensure that it is properly stored.
2. Prepare a suitable buffer solution for the experiment, typically a Tris or phosphate buffer.
3. Dilute the Hsc70 Protein-ATPase Fragment in the buffer to the desired concentration.
4. If applicable, add ATP to the solution at the required concentration to activate the ATPase function.
5. Transfer the solution into appropriate reaction vessels, such as microcentrifuge tubes or cuvettes.
6. Incubate the sample under optimal temperature and conditions for the desired period.
7. Measure the ATPase activity using a suitable assay method, such as a colorimetric or luminescent assay.
8. Analyze the data obtained to assess the efficiency and effectiveness of the Hsc70 Protein-ATPase Fragment.

Who needs Hsc70 Protein-ATPase Fragment?

1. Researchers studying protein folding and chaperone mechanisms in cells.
2. Biochemists investigating ATPase activity for enzyme function characterization.
3. Pharmaceutical companies developing drugs targeting protein misfolding diseases.
4. Academic institutions conducting research in cellular stress response and heat shock proteins.

People Also Ask about

What is the role of Hsp70?

Hsp70 proteins are central components of the cellular network of molecular chaperones and folding catalysts. They assist a large variety of protein folding processes in the cell by transient association of their substrate binding domain with short hydrophobic peptide segments within their substrate proteins.

What is the role of the human heat shock protein Hsp70 in protection against stress induced apoptosis?

Hsp70, the major stress-inducible heat shock protein, has been shown to inhibit the stress-induced JNK signaling pathway (21, 22), cytochrome c release (23, 24), apoptosome formation (25, 26), and caspase activation (21, 27) as well as events that are independent of caspase activation, including prevention of lysosomal

Is HSC70 a chaperone?

Heat shock cognate protein 70 (HSC70) is a constitutively expressed molecular chaperone which belongs to the heat shock protein 70 (HSP70) family. HSC70 shares some of the structural and functional similarity with HSP70.

What do Hsp70 proteins do?

The Hsp70 chaperones participate in a wide range of cellular housekeeping functions (top), including the folding of newly synthesized proteins, the translocation of polypeptides into mitochondria, chloroplasts and the endoplasmic reticulum (ER), the assembly and disassembly of protein complexes, regulation of protein

What is the function of the HSC70 protein?

In conjunction with cochaperones, Hsc70 removes clathrin from vesicles after they pinch off the plasma membrane. Hsc70 also plays a role in nucleocytoplasmic transport by shuttling across the nuclear membrane in an ATP-dependent manner and helping to export and recycle the nuclear import receptor importin.

What is the ATPase activity of Hsp70?

A general property of Hsp70 proteins is the ability of the ATPase activity to be stimulated by hydrophobic peptides and unfolded proteins (4). As reported here, the ATPase activity of Ssb could not be stimulated by peptides or unfolded proteins that were able to stimulate other Hsp70s of yeast.

What is the function of HSC70?

In conjunction with cochaperones, Hsc70 removes clathrin from vesicles after they pinch off the plasma membrane. Hsc70 also plays a role in nucleocytoplasmic transport by shuttling across the nuclear membrane in an ATP-dependent manner and helping to export and recycle the nuclear import receptor importin.

What is the function of HSP60 and Hsp70?

The Hsp60 and Hsp70 chaperone systems play a central role in the folding of matrix-localised proteins. In this article, we summarise major aspects of the molecular function of these nano-machines and their interplay with other matrix chaperones and proteases.

For pdfFiller’s FAQs

What is Hsc70 Protein-ATPase Fragment?

Hsc70 Protein-ATPase Fragment is a segment of the Hsc70 protein that possesses ATPase activity, involved in protein folding and stress response.

Who is required to file Hsc70 Protein-ATPase Fragment?

Researchers and professionals in the field of molecular biology and biochemistry who are studying protein transport, folding, or cellular stress responses may need to file Hsc70 Protein-ATPase Fragment data.

How to fill out Hsc70 Protein-ATPase Fragment?

To fill out Hsc70 Protein-ATPase Fragment documentation, one must follow specific protocols for data entry, including the protein's sequence, structural information, and experimental conditions.

What is the purpose of Hsc70 Protein-ATPase Fragment?

The purpose of Hsc70 Protein-ATPase Fragment is to facilitate protein folding, prevent misfolding, and assist in the transport of proteins across cellular membranes.

What information must be reported on Hsc70 Protein-ATPase Fragment?

Information reported must include the amino acid sequence, ATP binding properties, structural conformation, and any relevant experimental results related to its biological functions.