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This document provides technical specifications and information about the Mouse Anti-Hsp70/Hsc70 Monoclonal Antibody conjugated to alkaline phosphatase, including details on applications, specificity,
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How to fill out Anti-Hsp70/Hsc70: AP Conjugate
01
Begin by gathering the necessary materials including the Anti-Hsp70/Hsc70 antibody, AP conjugate, assay buffer, and a micropipette.
02
Prepare your sample by ensuring it is properly diluted in the assay buffer as per the manufacturer's instructions.
03
Add the required volume of Anti-Hsp70/Hsc70 to the diluted sample, typically a reaction volume specified in the protocol.
04
Incubate the sample at the recommended temperature for the specified time to allow for proper binding of the antibody.
05
Wash the sample gently with wash buffer to remove any unbound antibodies.
06
Add the AP conjugate to the sample, again using the specified volume from the protocol.
07
Incubate the mixture again following the time and temperature guidelines to ensure the conjugate attaches.
08
Perform additional washes to eliminate excess AP conjugate.
09
Proceed to detect the bound AP conjugate using a suitable substrate for alkaline phosphatase, following the assay conditions outlined in the protocol.
10
Analyze the results according to the method established for your assay.
Who needs Anti-Hsp70/Hsc70: AP Conjugate?
01
Researchers studying cellular stress responses or protein folding.
02
Clinicians involved in diagnosing diseases related to heat shock proteins.
03
Laboratories conducting basic or applied research on cancer, neurodegenerative diseases, or autoimmune disorders.
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What is Anti-Hsp70/Hsc70: AP Conjugate?
Anti-Hsp70/Hsc70: AP Conjugate is a laboratory reagent that consists of an antibody directed against the Heat Shock Protein 70 (Hsp70) or Heat Shock Cognate Protein 70 (Hsc70), conjugated to alkaline phosphatase (AP). It is used in immunological assays to detect the presence and quantify the levels of Hsp70/Hsc70 proteins in biological samples.
Who is required to file Anti-Hsp70/Hsc70: AP Conjugate?
Researchers and laboratory technicians utilizing this reagent in their experiments and studies are generally required to file reports regarding its use, especially when it pertains to clinical trials or regulatory submissions.
How to fill out Anti-Hsp70/Hsc70: AP Conjugate?
Filling out the Anti-Hsp70/Hsc70: AP Conjugate documentation typically involves providing details such as the batch number, expiration date, reagent concentration, storage conditions, and experimental conditions under which the reagent is used.
What is the purpose of Anti-Hsp70/Hsc70: AP Conjugate?
The purpose of the Anti-Hsp70/Hsc70: AP Conjugate is to facilitate the detection and quantification of Hsp70/Hsc70 proteins in various biological samples, aiding in research related to stress responses, cancer, and other diseases.
What information must be reported on Anti-Hsp70/Hsc70: AP Conjugate?
Information that must be reported includes the reagent's product identification details, batch number, usage instructions, manufacturer information, and any specific safety or handling precautions related to the reagent.
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