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Published April 1, 1987 3 HUMAN T CELL RECEPTOR V, GENE POLYMORPHISM BY PATRICK CONCANNON, RICHARD A. PATTI, * AND LEROY E. HOOD From the Division of Biology, California Institute of Technology, Pasadena,
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Obtain a human T cell receptor gene sequence from a reliable source or isolate it from a human sample.
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Amplify the gene sequence using polymerase chain reaction (PCR) to produce multiple copies of it.
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Clone the amplified gene sequence into an appropriate vector, such as a plasmid vector, using specific restriction enzymes and ligase.
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Transform the vector containing the cloned gene sequence into a suitable host organism, such as bacteria or yeast, to allow for replication and expression of the receptor.
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Cultivate and grow the transformed host organism to obtain a sufficient amount of the human T cell receptor protein.
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Researchers studying immunology and T cell biology require human T cell receptors to investigate their functions, interactions, and signaling pathways.
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The human T cell receptor (TCR) is a molecule found on the surface of T cells, a type of white blood cell, that is responsible for recognizing and binding to specific antigens.
There is no specific requirement for filing the human T cell receptor as it is a biological component present in human T cells.
The human T cell receptor does not need to be filled out as it is a naturally occurring molecule present in T cells.
The purpose of the human T cell receptor is to play a crucial role in the immune response by allowing T cells to recognize and respond to specific antigens, such as those found on pathogenic cells.
There is no specific information that needs to be reported on the human T cell receptor as it is a biological component.
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