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WHO Global Foodborne Infections Network (formerly WHO Global Salisbury) “A WHO network building capacity to detect, control and prevent foodborne and other enteric infections from farm to table

How to fill out susceptibility testing of salmonella

How to fill out susceptibility testing of salmonella:

1. Start by collecting the sample: Obtain a sample of the suspected salmonella strain from the patient or the infected source. This could be a stool sample, blood sample, or any other appropriate specimen.
2. Preparing the agar plates: Use a sterile loop or swab to streak the salmonella sample onto appropriate agar plates. These plates can contain selective media specific for salmonella growth.
3. Incubation period: Place the agar plates in an incubator at the optimal temperature for salmonella growth. This is usually around 37 degrees Celsius. Allow the plates to incubate for a specific period of time, such as 24-48 hours.
4. Interpretation of results: After the incubation period, assess the growth on the agar plates. Look for characteristic colonies resembling salmonella. Confirm the presence of salmonella through Gram staining and other biochemical tests, if required.
5. Antibiotic susceptibility testing: Using the pure culture obtained from the agar plates, perform antibiotic susceptibility testing. This involves placing antibiotic discs on the agar plates and measuring the zone of inhibition around each disc to determine the susceptibility or resistance of the salmonella strain to different antibiotics.
6. Record the results: Record the diameter of the zone of inhibition for each antibiotic tested. Compare these results to established interpretive criteria to determine if the strain is susceptible, intermediate, or resistant to each antibiotic.
7. Reporting the results: Compile the susceptibility testing results into a comprehensive report. Include the identified salmonella strain, the antibiotics tested, and their corresponding susceptibility/resistance outcomes. This report will help guide appropriate treatment options.

Who needs susceptibility testing of salmonella:

1. Individuals with suspected salmonella infection: Susceptibility testing is important to determine the most effective antibiotics for treating the infection. This information helps guide clinicians in selecting appropriate antimicrobial therapy.
2. Healthcare professionals: Susceptibility testing is essential for healthcare professionals involved in diagnosing and treating salmonella infections. It provides them with valuable information about which antibiotics are most likely to be effective in treating the specific strain of salmonella.
3. Public health agencies: Susceptibility testing of salmonella strains helps public health agencies monitor antibiotic resistance patterns. This information is crucial for tracking the emergence and spread of drug-resistant strains, facilitating appropriate interventions and control measures.

For pdfFiller’s FAQs

What is susceptibility testing of salmonella?

Susceptibility testing of salmonella is a process used to determine the effectiveness of antibiotics in treating infections caused by salmonella bacteria.

Who is required to file susceptibility testing of salmonella?

Healthcare providers and laboratories are required to file susceptibility testing of salmonella results.

How to fill out susceptibility testing of salmonella?

Susceptibility testing of salmonella can be filled out by documenting the results of antibiotic effectiveness on specific strains of the bacteria.

What is the purpose of susceptibility testing of salmonella?

The purpose of susceptibility testing of salmonella is to guide healthcare providers in choosing the most effective antibiotics for treating infections caused by salmonella.

What information must be reported on susceptibility testing of salmonella?

The report must include the specific strain of salmonella tested, the antibiotics used, and the level of effectiveness of each antibiotic.

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