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013 5. 726 0. 011 5. 741 0. 037 time Figure 2 Calibration Curve of the Pomalidomide PML by RP-HPLC Figure 3 Typical Chromatogram of Pomalidomide Standard by RP-HPLC CONCLUSION A simple and easily available HPLC method was developed in this study for the quantification of PML in its simplicity. The results of the study showed that the proposed RP-HPLC method is simple rapid precise and accurate which is useful for the routine determination of PML in bulk drug and in its pharmaceutical dosage...

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Well then who's the different components here in the HPLC alright so again come here we have our what are these solvents something else it's solvents all right why are there multiple bottles because you have different percentages depending on your extract per your HPLC method okay I mean my method might say I want 10% of site nitrite, but I can make a solution that was 10% of nitro right so what is the advantage of having two bottles so that we don't have to mix of the shuns over and over again alright, but the real reason is that you can do Brady where you can alter the solution concentration over time right because the pumps can control there are two pumps and a mixer where you can slowly change the concentration on the gradient elation it didn't look like anybody had to do that, but that's why we have those solvent it also can facilitate arrives from trying different ratios we don't want to be making solution to our solids up here we have four different solvents a1 b1 a2 b2 four different tubes come down into the mixer here or sorry T gas the Gasser removes bubbles, but it doesn't remove a big visible bubbles it just removes the microscopic small ones which are very problematic for columns and so in the past you would have to dig ass all of your solvents before you used in any LC method any else the instrumentation so this D gas is very valuable so the D gas removes very small bubbles and then the solvents come into this area here where they're full but to Mars okay, so we want to verify whatever solvents we're going to be using that there are no bubbles in those lines in this life in Miami — but this is back here the 5% ethanol solution, so these are our two pumps again right here this these are the two the four spots where we can open up the tubing and allow air bubbles to come out to purge the lines in this all right when you do that you want to make sure you don't let water fall into this thing I hear you guys remember this little does the sensor it's a sensor for lease bill right so if there's some sort of leak it wants to shut it down for you so you don't waste your time because there's a leak it's not doing what he thinks you's not what you think it's doing and there are many spots where it will detect a leak and different components this little bow here do you guys know what this is it directs it yeah in to raise it you can either go right to the waste if it's lefty loose right then it'll never go to the call it'll just go right to the waste all right that's generally how you would start things up because you don't know if there are bubbles in these lines, and you want to make sure there's no okay right tight again we'll make the flow go over the column all right this is our auto sampler area and down here is it is says therm I think it's really these two components down here are the detectors this one is our visible and UV detector there's our visible bulb and here's our new sorry over visible goal our UV bulb in the visible bulb and...

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What is hplc method for pomalidomide?

HPLC method for pomalidomide is a high-performance liquid chromatography method used to analyze and quantify pomalidomide in a sample.

Who is required to file hplc method for pomalidomide?

The pharmaceutical companies or researchers developing and testing pomalidomide are required to file the HPLC method for pomalidomide.

How to fill out hplc method for pomalidomide?

To fill out the HPLC method for pomalidomide, relevant information such as sample preparation technique, mobile phase composition, instrument parameters, and detection wavelength should be provided.

What is the purpose of hplc method for pomalidomide?

The purpose of the HPLC method for pomalidomide is to accurately determine the concentration and purity of pomalidomide in a given sample.

What information must be reported on hplc method for pomalidomide?

The HPLC method for pomalidomide should include information such as sample name, sample preparation details, instrument details, chromatographic conditions, and calculations used for quantification.