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Alright let#39’s recap a typical real-time or PCR experiment in a plate what you will require is the actual plate the plastics and the plastic holder the optical adhesive cover and the optical adhesive sealer in addition for the reagents if you#39’re doing a tag line experiment you will require the tech man fast advance master mix attack man gene expression assay cyber experiments will require the cyber green master mix and two primers which I have designated primer one in primer 2 P 1 and P 2 alsoyou'’ll require your C DNA sample and finally some water these pipette men adhere to show you that you essentially pipette the reagents into the plate you then seal the plate this is the final product which we then run on the instrument and in this case the step 1plus and now IN#39’m walking over to the step 1 plus IN#39’m going to load my platen the instrument simply by pulling open the door and setting the plate the goes up in the top as usual and closing the door now IN#39’m just going to use the touch screen function of the step 1 plus and I already have each man-fast cDNAprotocol right here on the main menu soil#39’m just going to choose that one and press save okay I saved my experiment IN#39’m going to start the run now say okay now what#39;happening is the cover is heating and bassoon as the cover has come up to the proper temperature you will see that this wideband becomes smaller as the plate is moving upland as it says here on the screen the required run temperature of the heated lid is 105 or right now at ninety point five so after a few more degrees the run will begin so now that the run is completed on the step one plus IN#39’m going to collect results by putting in my Drive and hitting the collect results button the system is busy it#39’s writing the USB please done#39’t remove the USB device, and you can see as the data visaing written we have the little bars coming across the screen now my experiments ready I may remove my USB storage I said okay my data is on herein#39’m going to go take it to my computerate my desk and analyze my results
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