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Once the pregnancy dipstick tests learning objectives the student is supposed to know the biochemical principles behind pregnancy tests and also be able to describe the test and nor the clinical application of the test a pregnancy test attempts to determine whether a female is pregnant pregnancy tests look for chemical markers associated with pregnancy these markets are found in urine and blood and pregnancy tests require sampling one of these substances principle the test utilizes a combination of antibodies including a monoclonal HCG antibody to selectively detect elevated levels of HCG the assay is conducted by immersing the test strip in a urine specimen and observing the formation of colored lines the specimen migrates via capillary action along the membrane to react with the colored conjugate the one-step pregnancy dipstick test involves the use of a one-step pregnancy test strip you the test strip is dipped in valuing the sample till a guess — the blue mark you the test device is then allowed to develop you interpretation of results for a negative result one red line appears in the control Regency and no apparent red or pink line appears in the test region T you for a positive result — distant red lines appear one line should be in the control Regency and another line should be in the test region t note the intensity of the red color in the test region t will vary depending on the concentration of HCG present in the specimen therefore any shade of red in the test region T should be considered positive one invalid result control line fails to appear this could be as a result of insufficient specimen volume or incorrect procedural techniques in such a situation review the procedure and repeat the test with a new test strip if the problem persists discontinue using the test kit immediately and contact your local distributor
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