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Instructions for Use Slab ELISA Enzyme immunoassay for the quantitative and qualitative determination of antibodies against soluble liver antigen (SLA) in human serum. RE70791 96 28 C I B L I N T
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How to fill out sla-ab elisa - nmlby
How to fill out SLA-AB ELISA - NMLBY:
01
Start by gathering all the necessary materials and reagents for the SLA-AB ELISA test.
02
Carefully read and understand the instructions provided with the SLA-AB ELISA kit.
03
Prepare the samples to be tested according to the specified protocol, ensuring proper labeling and identification.
04
Set up the ELISA plate by adding the appropriate amount of capture antibody to each well.
05
Incubate the plate at the appropriate temperature and time as instructed to allow the capture antibody to bind to any SLA-AB present in the samples.
06
After incubation, wash the plate thoroughly to remove any unbound substances.
07
Add the properly diluted detection antibody to each well and incubate again at the specified conditions to allow binding to occur.
08
Wash the plate again to remove any unbound detection antibodies.
09
Add the enzyme-conjugated secondary antibody to each well and incubate once more to allow for the formation of immune complexes.
10
Perform another round of washing to eliminate any unbound enzyme-conjugated secondary antibodies.
11
Add the substrate solution to each well, and incubate for the recommended duration to initiate the enzyme-substrate reaction.
12
Stop the reaction by adding the stop solution and mix gently.
13
Measure the optical density (OD) of each well using a microplate reader and record the results.
14
Analyze the data and interpret the SLA-AB ELISA test results based on the provided guidelines.
Who needs SLA-AB ELISA - NMLBY:
01
Individuals suspected of having autoimmune disorders, particularly systemic lupus erythematosus (SLE).
02
Researchers studying autoimmune diseases and investigating the presence of specific antibodies.
03
Clinicians and healthcare professionals involved in diagnosing and monitoring patients with autoimmune conditions.
04
Laboratories performing immunological testing for autoimmune markers, including SLA-AB.
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