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Rat Kim1 ELISA Cat. No.: RRBA001R 1. BACKGROUND Kim1 is a type I transmembrane structural glycoprotein located in the renal proximal tubule epithelial cells. These cells undergo regeneration after
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How to fill out rat kim-1 elisa
How to fill out rat kim-1 elisa:
01
Start by gathering all the necessary materials for the rat kim-1 elisa test, which typically include the kit components, the rat samples, and any reagents or buffers required.
02
Ensure that you have read and understood the instructions provided in the kit manual or protocol for the rat kim-1 elisa.
03
Prepare the samples by following the recommended guidelines, which may involve diluting or treating the rat samples using specific techniques or reagents mentioned in the protocol.
04
Label the microplate wells according to the provided template, including controls and standards as specified by the protocol.
05
Add the prepared samples into the designated wells of the microplate according to the specific volume indicated in the protocol.
06
Proceed to add the appropriate reagents or buffers to each well, as instructed.
07
Carefully cover the microplate with a plate sealer or lid to prevent contamination and ensure the reactions take place undisturbed.
08
Incubate the microplate for the recommended duration, typically at a specified temperature and in the given conditions mentioned in the protocol.
09
After the incubation period, remove the plate seal or lid and discard any remaining liquid from the wells by inverting the plate over an appropriate container and gently tapping it on absorbent paper.
10
Follow the next steps mentioned in the protocol, which often include washing the wells to remove any unbound substances and adding substrates for the colorimetric or fluorescent detection of kim-1.
11
Perform the required measurements using a suitable imaging or detection system, following the provided guidelines in the kit protocol.
12
Finally, analyze and interpret the results obtained from the rat kim-1 elisa according to the provided instructions and reference values to determine the kim-1 levels in the rat samples.
Who needs rat kim-1 elisa:
01
Researchers studying kidney diseases: Rat kim-1 elisa can provide valuable insights into kidney injury and diseases by measuring the levels of the kidney injury molecule-1 (kim-1) in rat samples. This information aids in understanding the pathogenesis, progression, and potential therapies for various kidney-related conditions.
02
Pharmacologists or drug developers: Rat kim-1 elisa can be used to assess the effects of drugs or potential therapeutic agents on kidney health and function. It helps in evaluating the nephrotoxicity or renal toxicity of drugs and monitoring their impact on kim-1 levels in rat models.
03
Toxicologists or environmental researchers: Rat kim-1 elisa can be utilized to investigate the impact of toxic substances, pollutants, or environmental factors on kidney injury and function in rat models. It allows for the assessment of kim-1 levels as a biomarker for renal damage caused by such exposures.
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What is rat kim-1 elisa?
Rat Kim-1 ELISA is a test used to measure kidney injury molecule-1 levels in rats.
Who is required to file rat kim-1 elisa?
Researchers conducting studies involving rats and kidney injury may be required to file Rat Kim-1 ELISA.
How to fill out rat kim-1 elisa?
To fill out Rat Kim-1 ELISA, one must follow the instructions provided by the manufacturer of the test kit.
What is the purpose of rat kim-1 elisa?
The purpose of Rat Kim-1 ELISA is to assess kidney injury in rats.
What information must be reported on rat kim-1 elisa?
The test results indicating the levels of kidney injury molecule-1 in rats must be reported on Rat Kim-1 ELISA.
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