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AFP (Alphafetoprotein) Elisa CAT NO DESCRIPTION PACK SIZE AFP Elisa 96 Tests EIAAFP1 Intended Use: AFP Elisa is intended to be used for the quantitative determination of AFP in Human serum. This reagent
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How to fill out afp alpha-fetoprotein elisa

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How to fill out AFP alpha-fetoprotein ELISA:

01
Begin by gathering all the necessary materials for the ELISA test, including the ELISA kit, samples, reagents, and a microplate reader.
02
Dilute the samples according to the instructions provided with the ELISA kit. This may involve using specific buffer solutions and following precise ratios.
03
Follow the manufacturer's instructions to prepare the microplate. This typically involves adding the diluted samples to the designated wells in the microplate, as well as adding controls and standards.
04
Incubate the microplate at the recommended temperature for the specified time period. This allows the AFP alpha-fetoprotein antigen and antibodies to react and form complexes.
05
After incubation, wash the microplate thoroughly to remove any unbound substances. Typically, this involves adding a wash buffer and gently tapping or aspirating the liquid from the wells.
06
Add the enzyme conjugate to the microplate, ensuring it is distributed evenly across all wells. Incubate the microplate again to allow binding between the conjugate and any remaining AFP alpha-fetoprotein complexes.
07
Repeat the washing step to remove unbound enzyme conjugate.
08
Add the substrate solution to the microplate, which initiates a color change reaction in wells containing the AFP alpha-fetoprotein complex.
09
Use a microplate reader to measure the optical density (OD) of each well at the appropriate wavelength specified in the instructions. Record the readings for further analysis.
10
Finally, interpret the results by comparing the OD values of the samples to the standards. The higher the OD value, the higher the concentration of AFP alpha-fetoprotein in the sample.

Who needs AFP alpha-fetoprotein ELISA?

01
AFP alpha-fetoprotein ELISA is commonly used in clinical laboratories and medical facilities.
02
It is primarily used to detect and measure the levels of AFP alpha-fetoprotein, a biomarker associated with certain types of cancer, particularly liver cancer.
03
The test may be ordered by physicians to screen for liver cancer or monitor the effectiveness of treatment in cancer patients.
04
AFP alpha-fetoprotein ELISA can also be useful in assessing the risk of neural tube defects during pregnancy or detecting other fetal abnormalities.
05
Researchers and scientists may also utilize AFP alpha-fetoprotein ELISA in their studies related to cancer, developmental biology, or other relevant areas of research.
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AFP alpha-fetoprotein ELISA is a test used to measure the levels of alpha-fetoprotein in the blood, which may be elevated in certain medical conditions like liver cancer or pregnancy.
Medical professionals or laboratories performing the test may be required to file the results of the AFP alpha-fetoprotein ELISA.
The AFP alpha-fetoprotein ELISA is typically filled out by entering the patient's information and the results of the test into a designated form or software.
The purpose of the AFP alpha-fetoprotein ELISA is to detect and monitor conditions such as liver cancer or certain fetal abnormalities.
Information such as patient demographics, test results, and any relevant medical history must be reported on the AFP alpha-fetoprotein ELISA form.
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