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Introduction Dithiothreitol DTT Cleland s reagent 1 is a cheap non-odorous reducing agent 2 which is produced by a safe and environmentally friendly process 3. In fact DTT was recently used as a scavenger during large scale preparation of the HIV fusion inhibitor 4. We now report that DTT reacts with trifluoroacetic acid TFA to form a bicyclic dithioorthoester which can be difficult to detect by HPLC.
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