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Journal of Experimental Botany, Vol. 49, No. 326, pp. 1481 1490, September 1998 Antisense-mediated suppression of transgene expression targeted specifically to pollen Joy E. Wilkinson1, Keith Lindsey2
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How to fill out antisense-mediated suppression of transgene
How to fill out antisense-mediated suppression of transgene?
01
First, prepare the antisense oligonucleotide that corresponds to the target gene sequence. This can be done through synthetic DNA oligonucleotide synthesis.
02
Next, introduce the antisense oligonucleotide into the cells or organisms of interest. This can be accomplished through various methods such as transfection, microinjection, or viral delivery.
03
Allow sufficient time for the antisense oligonucleotide to hybridize with the complementary mRNA molecule of the target gene.
04
The hybridization of the antisense oligonucleotide with the target mRNA leads to the formation of a double-stranded RNA molecule.
05
The double-stranded RNA triggers the RNA interference (RNAi) pathway, leading to the degradation of the target mRNA molecule.
06
As a result, the expression of the target gene is suppressed, leading to decreased levels of the corresponding protein.
Who needs antisense-mediated suppression of transgene?
01
Researchers studying gene function and regulation often utilize antisense-mediated suppression of transgene to investigate the consequences of gene downregulation.
02
Antisense-mediated suppression of transgene can be used in biotechnology applications to fine-tune gene expression levels or silence specific genes of interest.
03
Antisense-mediated suppression of transgene can also be potentially used as a therapeutic approach for treating genetic diseases caused by overexpression of certain genes.
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What is antisense-mediated suppression of transgene?
Antisense-mediated suppression of transgene is a method used to downregulate the expression of a specific transgene by introducing complementary RNA strands that bind to the messenger RNA of the transgene, inhibiting its translation and thereby reducing its protein production.
Who is required to file antisense-mediated suppression of transgene?
The individuals or organizations conducting research or experiments involving transgenes and utilizing antisense-mediated suppression methods are required to file the respective documentation.
How to fill out antisense-mediated suppression of transgene?
To fill out the antisense-mediated suppression of transgene documentation, the required information must be provided accurately and completely. This includes details about the transgene, the antisense sequence used, experimental methods, and any expected outcomes or potential risks associated with the suppression process.
What is the purpose of antisense-mediated suppression of transgene?
The purpose of antisense-mediated suppression of transgene is to study the effects of reducing or inhibiting the expression of specific transgenes. This method allows researchers to investigate the role and function of transgenes, assess their potential impacts, and understand how they contribute to biological processes.
What information must be reported on antisense-mediated suppression of transgene?
The documentation for antisense-mediated suppression of transgene should include information such as the identity of the transgene, the sequence of the antisense RNA used, the experimental method employed, any observed effects or outcomes, and a detailed description of the study design and objectives.
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