Get the free Padlock oligonucleotides for duplex DNA based on sequence ... - pnas

An oligonucleotide was circularized around double-stranded DNA thanks to triple helix formation. Short oligonucleotides are known to be able to form DNA triple ...

How to fill out padlock oligonucleotides for duplex

How to fill out padlock oligonucleotides for duplex:

1. Design the padlock oligonucleotide sequences specific to the target DNA region.
2. Order the oligonucleotides from a reputable DNA synthesis service.
3. Dissolve the oligonucleotides in a suitable buffer to obtain a desired concentration.
4. Anneal the oligonucleotides by heating the solution and slowly cooling it down to allow the individual strands to come together and form a duplex.
5. Verify the annealing efficiency by running an agarose gel electrophoresis or using other appropriate methods.
6. Store the duplex padlock oligonucleotides at the recommended temperature to maintain their stability.

Who needs padlock oligonucleotides for duplex:

1. Researchers conducting genetic research and sequencing studies can utilize padlock oligonucleotides for duplex formation.
2. Laboratories investigating specific target DNA regions in different biological samples often employ padlock oligonucleotides for duplex applications.
3. Scientists studying the structure and function of DNA can benefit from padlock oligonucleotides for duplex analysis and manipulation.

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What is padlock oligonucleotides for duplex?

Padlock oligonucleotides for duplex is a technique used in molecular biology to accurately detect and amplify specific DNA sequences. It involves the ligation of two short DNA sequences called probes to form a circular DNA molecule, known as a padlock. This padlock is then amplified and used to identify and quantify the presence of a target DNA sequence in a sample.

Who is required to file padlock oligonucleotides for duplex?

In the context of molecular biology research, there is no specific requirement to file padlock oligonucleotides for duplex. However, researchers or scientists conducting molecular biology experiments or studies may utilize padlock oligonucleotides for duplex as a technique to detect and amplify specific DNA sequences.

How to fill out padlock oligonucleotides for duplex?

The process of filling out padlock oligonucleotides for duplex involves the design and synthesis of specific DNA probes that can anneal to the target DNA sequences of interest. These probes are then ligated together in a circular manner using DNA ligase. The resulting padlock structures can be further amplified using various techniques such as polymerase chain reaction (PCR) and utilized for detecting and quantifying the target DNA sequences.

What is the purpose of padlock oligonucleotides for duplex?

The purpose of padlock oligonucleotides for duplex is to accurately detect and quantify specific DNA sequences in a sample. It can be used in various molecular biology applications such as genetic testing, genotyping, and detecting mutations or variations in DNA sequences. This technique offers high specificity and sensitivity, making it valuable in research and diagnostic settings.

What information must be reported on padlock oligonucleotides for duplex?

The specific information that needs to be reported on padlock oligonucleotides for duplex may vary depending on the purpose and context of the experiment. However, typically, the sequence of the DNA probes, including the target binding region, any modifications or labels used on the probes, and details of the experimental conditions and methodology employed should be reported.

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