Get the free MOUSE SOLUBLE CD36 ELISA KIT - Aviscera Bioscience

MOUSE SOLUBLE CD36 ELISA KIT Page 1 MOUSE SOLUBLE CD36 ELISA KIT PURCHASE INFORMATION: FOR THE QUANTITATIVE DETERMINATION OF MOUSE CD36 CONCENTRATIONS IN CELL CULTURE SUPERSTATES AND PLASMA. ELISA

How to fill out mouse soluble cd36 elisa

How to fill out mouse soluble CD36 ELISA:

1. Prepare the samples: Collect mouse plasma or other biological samples to be tested for soluble CD36. Centrifuge the samples to remove any debris or cells. Dilute the samples to the appropriate concentration if necessary.
2. Prepare the ELISA plate: Coat the wells of a microplate with a capture antibody specific for soluble CD36. Incubate the plate overnight at a recommended temperature to allow the capture antibody to bind to the CD36.
3. Wash the plate: After the incubation, wash the plate thoroughly to remove any unbound materials. Use an appropriate buffer solution and ensure complete removal of any residual liquid in the wells.
4. Block the nonspecific binding sites: Add a blocking buffer to the wells to prevent any further nonspecific binding. Incubate the plate for a recommended time to ensure complete blocking.
5. Add the samples: Pipette the prepared samples into the wells of the ELISA plate. Include appropriate positive and negative control samples for comparison purposes. Incubate the plate for a specific time to allow the soluble CD36 to bind to the capture antibody.
6. Wash the plate again: After the incubation, wash the plate thoroughly to remove any unbound samples or antibodies.
7. Add the detection antibody: Add a detection antibody specific for soluble CD36 to each well. Incubate the plate for a recommended time to allow the detection antibody to bind to the CD36.
8. Wash the plate once more: After the incubation, wash the plate thoroughly to remove any unbound detection antibody.
9. Add the substrate: Add a substrate solution that will produce a measurable signal upon enzymatic reaction with the bound detection antibody. Incubate the plate for a specific time to allow the reaction to occur.
10. Stop the reaction: Add a stop solution to halt the enzymatic reaction and stabilize the signal generated.
11. Measure the absorbance: Use a microplate reader to measure the absorbance of each well at the appropriate wavelength. This will provide quantitative data on the concentration of soluble CD36 in each sample.

Who needs mouse soluble CD36 ELISA:

1. Researchers studying the role of soluble CD36 in mouse models: Mouse soluble CD36 ELISA can provide valuable information about the concentration of soluble CD36 in different samples, allowing researchers to better understand its function and potential implications in various biological processes.
2. Pharmaceutical companies developing CD36-targeted therapies: Mouse soluble CD36 ELISA can be used to assess the efficacy of CD36-targeted drugs or therapies in preclinical studies. Monitoring the levels of soluble CD36 can help evaluate the impact of the treatment on CD36 modulation.
3. Biomedical scientists investigating CD36-related diseases: Research focused on diseases or conditions where CD36 dysregulation is implicated may require mouse soluble CD36 ELISA to analyze the levels of soluble CD36 in various biological samples. This can help identify potential biomarkers or therapeutic targets associated with CD36-related pathologies.
4. Veterinary researchers studying CD36 in animal health: Veterinarians and animal health researchers may utilize mouse soluble CD36 ELISA to investigate the role of CD36 in specific animal species or diseases. This can aid in understanding the functional significance of CD36 in veterinary medicine and identifying potential diagnostic or therapeutic applications.

For pdfFiller’s FAQs

What is mouse soluble cd36 elisa?

Mouse soluble cd36 elisa is an enzyme-linked immunosorbent assay used to measure the levels of soluble CD36 in mouse samples.

Who is required to file mouse soluble cd36 elisa?

Researchers or scientists conducting studies or experiments involving mouse samples may be required to file mouse soluble cd36 elisa.

How to fill out mouse soluble cd36 elisa?

To fill out mouse soluble cd36 elisa, one needs to carefully follow the instructions provided in the protocol or kit manual, and accurately record the experimental data.

What is the purpose of mouse soluble cd36 elisa?

The purpose of mouse soluble cd36 elisa is to quantify the levels of soluble CD36 protein in mouse samples, which can provide insights into various biological processes.

What information must be reported on mouse soluble cd36 elisa?

Information such as sample identification, dilution factors, absorbance values, standard curve data, and calculations must be reported on mouse soluble cd36 elisa.

How do I execute mouse soluble cd36 elisa online?

pdfFiller has made it simple to fill out and eSign mouse soluble cd36 elisa. The application has capabilities that allow you to modify and rearrange PDF content, add fillable fields, and eSign the document. Begin a free trial to discover all of the features of pdfFiller, the best document editing solution.

How do I fill out the mouse soluble cd36 elisa form on my smartphone?

Use the pdfFiller mobile app to complete and sign mouse soluble cd36 elisa on your mobile device. Visit our web page (https://edit-pdf-ios-android.pdffiller.com/) to learn more about our mobile applications, the capabilities you’ll have access to, and the steps to take to get up and running.

How do I complete mouse soluble cd36 elisa on an Android device?

Use the pdfFiller mobile app and complete your mouse soluble cd36 elisa and other documents on your Android device. The app provides you with all essential document management features, such as editing content, eSigning, annotating, sharing files, etc. You will have access to your documents at any time, as long as there is an internet connection.