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HUMAN SOLUBLE CD163 ELISA KIT PAGE 1 HUMAN SOLUBLE CD163 ELISA KIT PURCHASE INFORMATION: FOR THE QUANTITATIVE DETERMINATION OF HUMAN SCD163 CONCENTRATIONS IN CELL CULTURE SUPERSTATES, EDTA PLASMA

How to fill out human cd163 elisa

To fill out the human CD163 ELISA, follow these steps:

1. Prepare the necessary materials and reagents for the assay, including the human CD163 ELISA kit, sample diluent, wash buffer, substrate solution, and stop solution.
2. Label the microtiter plate wells according to the provided instructions. Typically, the wells are labeled for standards, samples, and controls.
3. Dilute the standards and controls using the sample diluent provided. Follow the instructions in the kit to determine the appropriate dilution factors.
4. Add the diluted standards, samples, and controls to their respective labeled wells in the microtiter plate. Make sure to use the appropriate volume for each well.
5. Cover the plate with a plate sealer or plastic film and incubate at the recommended temperature and time. This allows for the antigen and antibody to bind.
6. After the incubation period, discard the liquid from the wells and wash the plate with the provided wash buffer. Repeat this step multiple times to remove any unbound substances.
7. Add the enzyme-linked antibody to each well, ensuring proper pipetting technique and avoiding cross-contamination.
8. Incubate the plate again according to the provided instructions to allow the formation of specific immune complex.
9. Wash the plate once more to remove any unbound enzyme-linked antibody.
10. Add the substrate solution to each well and incubate again for the recommended time. This initiates a color reaction, which will eventually change the well color.
11. Stop the enzyme reaction by adding the stop solution to each well. This halts the color development and stabilizes the results.
12. Measure the absorbance of each well at the appropriate wavelength using a microplate reader.

Who needs human CD163 ELISA?

Human CD163 ELISA is typically used by researchers, scientists, and medical professionals who study or work with the CD163 protein. This protein is primarily found on the surface of macrophages and is involved in a range of functions such as inflammation regulation, immune response modulation, and clearance of hemoglobin-haptoglobin complexes. Studying CD163 using ELISA can provide valuable insights into various diseases or conditions where this protein is implicated, including certain inflammatory disorders, cancer, and infectious diseases. Additionally, the measurement of CD163 levels through ELISA can be used for diagnosis, prognosis, and monitoring of therapeutic interventions in specific patient populations.

For pdfFiller’s FAQs

What is human cd163 elisa?

Human CD163 ELISA is a test used to measure the levels of CD163 protein in the blood, which can be an indicator of certain medical conditions.

Who is required to file human cd163 elisa?

Medical professionals or researchers conducting studies that require analysis of CD163 protein levels may be required to perform and file human CD163 ELISA.

How to fill out human cd163 elisa?

To fill out a human CD163 ELISA, follow the specific instructions provided with the test kit. Generally, it involves adding samples, reagents, and conducting measurements according to the protocol.

What is the purpose of human cd163 elisa?

The purpose of human CD163 ELISA is to quantify the levels of CD163 protein in blood samples for diagnostic or research purposes.

What information must be reported on human cd163 elisa?

The results of the CD163 protein levels in blood samples, including the units of measurement, any controls used, and any relevant sample information.

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