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HUMAN SOLUBLE ICAM1 ELISA KIT PAGE 1 HUMAN SOLUBLE INTRACELLULAR CELL ADHESION MOLECULE1 (ICAM1) ELISA KIT FOR THE QUANTITATIVE DETERMINATION OF HUMAN SOLUBLE ICAM1 CONCENTRATIONS IN SERUM, PLASMA
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How to fill out human soluble icam-1 elisa
How to fill out human soluble ICAM-1 ELISA:
01
Start by preparing the necessary reagents and samples. Ensure that all reagents are properly mixed and equilibrated to room temperature.
02
Label the microplate wells according to the sample type and dilution factor. Include appropriate controls such as blank, standard, and quality control samples.
03
Add the diluted standards and samples to the respective wells in duplicate or triplicate, following the provided dilution instructions. Be careful not to cross-contaminate wells.
04
Incubate the plate for a specific period of time at a designated temperature. Typically, this is done by placing the plate in an incubator or shaking it gently on a microplate shaker.
05
After the incubation, thoroughly rinse the plate wells with a wash buffer to remove any unbound substances. Repeat this step multiple times according to the manufacturer's instructions.
06
Add the detection reagent (e.g., enzyme-conjugated antibody) to each well and gently mix. Incubate the plate again under the prescribed conditions.
07
Wash the plate as before to remove any unbound detection reagent.
08
Add the substrate solution to each well, ensuring proper mixing. Incubate the plate in the dark for a specified duration.
09
Stop the reaction by adding a stop solution, if provided by the manufacturer.
10
Measure the absorbance of each well using a microplate reader set to the appropriate wavelength. Record the values for further analysis.
11
Generate a standard curve using the standard absorbance values and the corresponding concentrations. Use this curve to determine the concentration of ICAM-1 in the samples.
12
Finally, analyze and interpret the results based on the provided guidelines or using appropriate statistical methods.
Who needs human soluble ICAM-1 ELISA?
01
Researchers studying inflammatory diseases and conditions involving immune responses may utilize human soluble ICAM-1 ELISA to assess levels of soluble ICAM-1 in various biological samples.
02
Clinicians and healthcare professionals may use this assay to aid in the diagnosis and monitoring of certain diseases, such as multiple sclerosis, rheumatoid arthritis, and cardiovascular disorders, where ICAM-1 levels have been implicated.
03
Pharmaceutical companies and drug developers might employ human soluble ICAM-1 ELISA to evaluate the efficacy of potential therapeutic interventions targeting ICAM-1 or to screen for novel compounds that modulate ICAM-1 expression or function.
04
Veterinary researchers investigating inflammatory conditions in animals may also find value in using human soluble ICAM-1 ELISA, as the assay can often be adapted for use with animal samples.
05
Additionally, academic institutions, biotechnology firms, and other scientific organizations studying cell adhesion molecules and their role in various physiological processes may utilize this assay in their research efforts.
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What is human soluble icam-1 elisa?
Human soluble ICAM-1 ELISA is a test used to quantify the levels of soluble intercellular adhesion molecule 1 in human samples.
Who is required to file human soluble icam-1 elisa?
Researchers, scientists, or healthcare professionals working with human samples may be required to file human soluble ICAM-1 ELISA results.
How to fill out human soluble icam-1 elisa?
To fill out a human soluble ICAM-1 ELISA, follow the instructions provided in the ELISA kit and accurately record the absorbance values.
What is the purpose of human soluble icam-1 elisa?
The purpose of human soluble ICAM-1 ELISA is to measure the levels of ICAM-1 in human samples, which can provide valuable information about immune response and inflammation.
What information must be reported on human soluble icam-1 elisa?
The human soluble ICAM-1 ELISA report should include the sample ID, absorbance values, standard curve, and calculated ICAM-1 concentrations.
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