Get the free Human soluble CD117 elisa kit - Aviscera Bioscience

HUMAN SOLUBLE CD117×KIT ELISA KIT PAGE 1 HUMAN SOLUBLE CD117×KIT ELISA KIT PURCHASE INFORMATION: Catalog No. HUMAN SOLUBLE CD117×KIT ELISA SK0066201 Formulation 96 T Standard Range 23 1500 pg×ml

How to fill out human soluble cd117 elisa

How to fill out human soluble CD117 ELISA:

1. Prepare the necessary reagents and samples by following the manufacturer's instructions. This typically includes thawing frozen samples, diluting standards, and labeling wells.
2. Add each standard, sample, and control into separate wells on the microplate according to the designated positions. Be sure to record the positions accurately.
3. Incubate the plate for the recommended time period at the appropriate temperature, allowing the soluble CD117 antigen to bind to the corresponding antibodies.
4. Wash the plate to remove any unbound substances. Be cautious to follow the washing steps precisely to avoid any missed wells or cross-contamination.
5. Add the detection antibody to each well and incubate once again to allow binding with the soluble CD117 antigen.
6. Wash the plate again to remove any excess detection antibody.
7. Add the appropriate substrate solution to each well and incubate, which will produce a color change in proportion to the amount of soluble CD117 present in the samples.
8. Stop the reaction by adding the stop solution as instructed and measure the optical density of each well using a microplate reader.
9. Use the absorbance values to plot a standard curve by plotting the concentration of the standards against their corresponding absorbance values.
10. Calculate the concentration of soluble CD117 in each sample based on their respective absorbance values using the standard curve equation provided by the manufacturer.

Who needs human soluble CD117 ELISA?

1. Researchers studying the expression and regulation of soluble CD117 in various biological samples may require human soluble CD117 ELISA.
2. Clinicians and scientists investigating the role of soluble CD117 in disease pathogenesis, such as cancer or allergic disorders, may benefit from using this ELISA kit.
3. Pharmaceutical companies developing therapies targeting the soluble CD117 pathway might use this assay to assess the effectiveness of their candidate drugs.
4. Immunologists researching cellular signaling and interactions involving the CD117 receptor and its soluble form may utilize this ELISA for their studies.
5. Diagnostics laboratories and medical professionals interested in assessing soluble CD117 levels in patient samples for diagnostic or prognostic purposes may utilize this ELISA kit.

For pdfFiller’s FAQs

What is human soluble cd117 elisa?

Human soluble cd117 ELISA is a type of enzyme-linked immunosorbent assay used to measure the amount of soluble CD117 protein in human samples.

Who is required to file human soluble cd117 elisa?

Researchers, scientists, or healthcare professionals studying CD117 protein levels in human samples may be required to file human soluble CD117 ELISA results.

How to fill out human soluble cd117 elisa?

To fill out human soluble CD117 ELISA, follow the instructions provided with the ELISA kit, including adding samples, standards, and controls to the wells, incubating, washing, and adding detection reagents.

What is the purpose of human soluble cd117 elisa?

The purpose of human soluble CD117 ELISA is to accurately quantify the levels of soluble CD117 protein in human samples, which can be useful in research, diagnosis, or monitoring of certain diseases.

What information must be reported on human soluble cd117 elisa?

Information such as the sample ID, CD117 protein concentration, standards used, controls, and any dilutions made must be reported on the human soluble CD117 ELISA results.

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