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HUMAN CTF ELISA KIT Page 1 HUMAN CONNECTIVE TISSUE GROWTH FACTOR (CTF) ELISA KIT PURCHASE INFORMATION: ELISA NAME HUMAN CTF ELISA Catalog No. SK0072602 Formulation 96 T Standard range Sensitivity
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How to fill out human ctgf elisa kit
How to fill out human CTGF ELISA kit:
01
Read the instructions carefully: Before starting, thoroughly read through the provided instructions that come with the human CTGF ELISA kit. Make sure you understand all the steps and requirements.
02
Prepare the necessary materials: Gather all the materials needed for the ELISA assay, such as the microplate, wash solution, sample diluent, standards, and controls. Ensure that they are all at the appropriate temperatures and ready for use.
03
Set up the microplate: Label the microplate wells according to the provided instructions. Add the required amount of standard, control, and sample solutions into the appropriate wells. Also, don't forget to include the blank well without any sample or standard.
04
Incubate and wash: Cover the microplate and incubate it for the specified time and temperature according to the instructions. After incubation, carefully remove the liquid from the wells and wash them gently with the provided wash solution.
05
Add the detection reagent: Add the detection reagent, which is usually an enzyme-linked antibody or substrate, into each well as instructed. Ensure that the reagent is properly distributed throughout the well by gently shaking or tapping the microplate.
06
Incubate and wash again: Cover the microplate and incubate it again for the specified time and temperature. After incubation, remove the liquid, wash the wells, and pat the microplate dry using a clean paper towel or tissue.
07
Add the substrate: Add the substrate solution to each well. This solution reacts with the enzyme-linked antibody or substrate and produces a color change. Allow sufficient time for the color to develop as specified in the instructions.
08
Stop the reaction: After the appropriate incubation time, add the stop solution to each well to halt the color development process. This will stabilize the color reaction and prevent further changes.
09
Measure the absorbance: Use a microplate reader to measure the absorbance of each well at the specific wavelength stated in the instructions. Record the readings for further analysis.
10
Analyze the results: Compare the absorbance values of the standards, controls, and samples to determine the concentration of CTGF present in the samples. Follow the provided guidelines or consult technical support if necessary.
Who needs human CTGF ELISA kit:
01
Research laboratories: Scientists and researchers studying CTGF and its role in various biological processes may require human CTGF ELISA kits to quantify the concentration of CTGF in different samples.
02
Pharmaceutical companies: Companies involved in drug development and testing may utilize human CTGF ELISA kits to assess the effects of potential drugs on CTGF expression and secretion.
03
Clinical diagnostics: Medical professionals and diagnostic laboratories may use human CTGF ELISA kits to measure CTGF levels in patient samples. This can assist in the diagnosis and monitoring of certain diseases or conditions associated with abnormal CTGF levels, such as fibrosis or certain cancers.
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What is human ctgf elisa kit?
Human CTGF ELISA kit is a tool used for the quantitative measurement of connective tissue growth factor in human samples.
Who is required to file human ctgf elisa kit?
Researchers, scientists, or healthcare professionals working with human samples interested in measuring connective tissue growth factor may use the human CTGF ELISA kit.
How to fill out human ctgf elisa kit?
Follow the instructions provided in the kit manual for proper sample preparation, assay procedure, and data analysis to fill out the human CTGF ELISA kit correctly.
What is the purpose of human ctgf elisa kit?
The purpose of the human CTGF ELISA kit is to accurately quantify the levels of connective tissue growth factor in human samples for research or clinical diagnostic purposes.
What information must be reported on human ctgf elisa kit?
The kit may require reporting details such as sample ID, standard curve values, absorbance readings, and calculated concentrations of human CTGF.
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