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CLIN. CHEM. 39/1 82-86 1993 HPLC of Folinic Acid Diastereoisomers and 5-Methyltetrahydrofolate Etienne Nathalie Speziale Marie-Christine and Gerard We present a rapid sensitive and automated HPLC method with direct resolution of I-folinic acid -FA d-fotinic acid d-FA and 5-methyltetrahydrofolate 5MTHF from plasma samples. 22. Van Tellingen 0 Van der Woude HR Benen JH Van Beers CJT Nooyen WJ. Stable and sensitive method for the simultaneous determ...
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How to fill out folinic acid hplc method
How to fill out folinic acid HPLC method:
01
Start by gathering all the necessary reagents and equipment for the HPLC method. This includes folinic acid reference standard, mobile phase solvents, HPLC columns, and a suitable HPLC instrument.
02
Prepare the mobile phase solution by mixing appropriate proportions of solvents, such as a mixture of water and acetonitrile. The exact composition may vary depending on the specific method or instrument used.
03
Set up the HPLC instrument according to the recommended parameters for folinic acid analysis. This usually involves selecting the appropriate column, adjusting the flow rate, and setting the detection wavelength.
04
Prepare the sample solution by dissolving the folinic acid sample in a suitable solvent or mobile phase. The concentration of the sample should be within the linear range of the HPLC method.
05
Inject the prepared sample solution into the HPLC instrument and begin the analysis. The instrument will separate and detect the folinic acid based on its retention time and absorbance at the selected wavelength.
06
Collect and analyze the HPLC data to determine the concentration of folinic acid in the sample. This can be done by comparing the peak area or height of the folinic acid peak with that of the reference standard.
Who needs folinic acid HPLC method:
01
Researchers or scientists involved in pharmaceutical development may need the folinic acid HPLC method to analyze the concentration and purity of folinic acid in drug formulations.
02
Clinical laboratories or medical professionals may require the folinic acid HPLC method to determine the level of folinic acid in patient samples for diagnostics or therapeutic drug monitoring.
03
Quality control personnel in manufacturing industries, such as pharmaceutical or nutraceutical companies, may utilize the folinic acid HPLC method to ensure the quality and consistency of their products containing folinic acid.
In summary, the folinic acid HPLC method is needed by various professionals and industries involved in pharmaceutical research, clinical diagnostics, and quality control to accurately analyze the concentration and purity of folinic acid.
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What is folinic acid hplc method?
Folinic acid HPLC method is a analytical technique used for the quantification of folinic acid in a sample using High Performance Liquid Chromatography (HPLC) technology.
Who is required to file folinic acid hplc method?
The individuals or organizations conducting research or analysis on folinic acid levels in samples are required to use and file the folinic acid HPLC method for reporting purposes.
How to fill out folinic acid hplc method?
To fill out the folinic acid HPLC method, one needs to follow the specified steps and guidelines provided by the regulatory authority or the relevant laboratory protocol. It typically involves sample preparation, setting up the HPLC instrument, running the analysis, and recording the results.
What is the purpose of folinic acid hplc method?
The purpose of the folinic acid HPLC method is to accurately and precisely measure the concentration of folinic acid in a given sample. This method is commonly used for quality control, research studies, or to verify the potency of folinic acid-containing pharmaceutical products.
What information must be reported on folinic acid hplc method?
The folinic acid HPLC method typically requires reporting various information, including but not limited to sample identification, HPLC column details, mobile phase composition, injection volume, retention time, peak area or height, calibration curve parameters, and calculated concentration of folinic acid in the sample.
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