Get the free HER-2/neu ELISA

HER2×new ELISA 06489876 INSTRUCTIONS FOR USE MODE EMPLOY Nuclear Diagnostic Laboratories LLC 100 Acorn Park Dr. 6th Floor Cambridge, MA 02140 USA TEL: 6174923900 www.oncogene.com Intended Use Principle

How to fill out her-2neu elisa

How to fill out her-2neu elisa:

1. Prepare the samples: Collect the necessary samples, such as tissue or blood, from the patient. Ensure proper handling and storage of the samples to maintain their integrity.
2. Dilute the samples: Depending on the specific elisa kit being used, appropriate dilutions of the samples may be required. Follow the instructions provided by the manufacturer to achieve the desired sample concentration.
3. Plate preparation: Prepare the ELISA plate by adding the necessary reagents or capture antibodies to the wells. Carefully follow the protocol provided with the kit to ensure proper coating of the plate.
4. Add the samples: Take the diluted samples prepared in step 2 and add them to the appropriate wells of the ELISA plate. Ensure accurate pipetting to prevent cross-contamination.
5. Incubation: Allow the samples to incubate in the wells for a specific period of time, usually as mentioned in the kit instructions. This allows for proper binding of the target molecule (HER-2neu) to the capture antibodies.
6. Wash the wells: After the incubation period, carefully wash the wells with the provided wash buffer to remove any unbound proteins or antibodies. This step helps to minimize background noise and enhance the accuracy of the assay.
7. Addition of detection antibodies: Add the detection antibodies or enzymes to the wells. These antibodies will specifically bind to the target HER-2neu protein if present, forming a sandwich with the capture antibodies.
8. Incubation and washing: Incubate the plate again for a specific period of time to allow sufficient binding between the detection antibodies and the target protein. Wash the wells again to remove any unbound detection antibodies.
9. Substrate addition: Add the substrate solution to the wells. This will initiate a reaction with the bound enzyme or detection antibodies, resulting in a color change or signal.
10. Read the results: Use a microplate reader to measure the optical density or signal generated in each well. Compare the obtained values with the provided standards or controls to determine the concentration or presence of HER-2neu in the samples.

Who needs her-2neu elisa:

1. Patients with breast cancer: HER-2neu elisa is commonly used in the diagnosis and monitoring of breast cancer. HER-2neu is a protein overexpressed in certain types of breast cancer, and detecting its presence or level can help determine the prognosis and guide treatment decisions.
2. Researchers and scientists: HER-2neu elisa is also used in research settings to study the biology and mechanisms of HER-2neu in various diseases, including cancer. Researchers may use this assay to investigate potential therapeutic targets or to understand the role of HER-2neu in different cellular processes.
3. Clinicians and oncologists: Clinicians and oncologists may use HER-2neu elisa to assess the effectiveness of targeted therapies, such as trastuzumab, in patients with HER-2 positive breast cancer. Regular monitoring of HER-2neu levels can help determine treatment response and guide personalized treatment strategies.

Overall, HER-2neu elisa plays a crucial role in the diagnosis, management, and research of breast cancer and other diseases involving the HER-2neu protein.

For pdfFiller’s FAQs

What is her-2neu elisa?

HER-2/neu ELISA is a laboratory test used to detect the presence of a protein called Human Epidermal Growth Factor Receptor 2 (HER-2/neu) in breast cancer cells.

Who is required to file her-2neu elisa?

Medical professionals and laboratories performing tests for breast cancer patients are required to conduct and report HER-2/neu ELISA.

How to fill out her-2neu elisa?

HER-2/neu ELISA is conducted by collecting a blood sample from the patient, processing it in the laboratory, and interpreting the results based on the presence of the HER-2/neu protein.

What is the purpose of her-2neu elisa?

The purpose of HER-2/neu ELISA is to help in the diagnosis, prognosis, and treatment planning for breast cancer patients.

What information must be reported on her-2neu elisa?

The HER-2/neu ELISA report must include the patient's information, test results, interpretation, and any relevant clinical recommendations.

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