Get the free Lab 6 Extracting DNA From Onions

Lab 6 Extracting DNA from Onions PROBLEM How can the DNA in onions be extracted? BACKGROUND The process of obtaining DNA from cells is the first step in much biotechnology laboratory procedures. Researchers

How to fill out lab 6 extracting dna

How to fill out lab 6 extracting DNA:

1. Gather all the necessary materials: You will need a test tube, a small amount of saliva or blood, a solution containing enzymes to break down the cell membranes, a substance to precipitate the DNA, and any additional tools mentioned in the lab protocol.
2. Label the test tube: Use a marker to clearly label the test tube with your name, the date, and the specific sample you are using (saliva or blood).
3. Prepare the sample: If using saliva, swab the inside of your mouth with a sterile cotton swab and place it into the test tube. If using blood, use a clean lancet to obtain a small drop of blood and transfer it into the test tube. Be careful to follow proper safety precautions and dispose of any sharps in designated containers.
4. Add the cell membrane-breaking enzymes: Using a pipette or dropper, carefully add the appropriate amount of the enzyme solution to the test tube containing the sample. Gently mix the contents by swirling or inverting the tube a few times.
5. Incubation: Place the test tube in a designated incubator or water bath, following the specific temperature and time requirements mentioned in the lab protocol. This step allows the enzymes to break down the cell membranes and release the DNA.
6. Precipitate the DNA: After the incubation period, add the precipitating substance to the test tube. This substance helps separate the DNA from other cellular components. Follow the instructions provided in the lab protocol for the specific precipitating substance and the amount to use.
7. Gently mix and observe: Carefully mix the contents of the test tube by inverting it a few times. You may notice the DNA precipitating and forming visible strands or clumps.
8. Extraction: Using a pipette or dropper, carefully transfer the supernatant (the liquid portion above the precipitated DNA) to another clean test tube. This step helps eliminate any remaining cellular debris or contaminants.
9. Collect the DNA: Using a clean pipette or dropper, carefully collect the precipitated DNA from the original test tube and transfer it into a separate container. Be meticulous to avoid any cross-contamination.
10. Store and analyze: Depending on the lab protocol, the extracted DNA may need to be stored in a specific solution or frozen for further analysis. Follow the instructions provided in the lab protocol for proper storage and analysis procedures.

Who needs lab 6 extracting DNA:

1. Students studying genetics or molecular biology: Lab 6 on extracting DNA is typically required as part of a biology curriculum, especially for those studying genetics or molecular biology. This lab helps students gain practical experience in DNA extraction techniques and understand the fundamental principles behind it.
2. Researchers in genetics or forensics: DNA extraction is a crucial first step in various research fields, including genetics and forensics. Researchers working in these areas often need to extract DNA from samples for further analysis and identification purposes.
3. Medical professionals: DNA extraction techniques are also relevant in the medical field, particularly in genetic testing and diagnosis. Medical professionals who work in genetics clinics or laboratories may need to perform DNA extraction to analyze and identify genetic abnormalities or inherited diseases in their patients.

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