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Transgenic 35S PSTOL1 plants and Affymetrix analysis. The PSTOL1 coding sequence was cloned into the binary vector pMDC32 with the 35S promoter and used for Agrobacterium-mediated transformation of immature IR64 and Nipponbare embryos. The construct was sequenced using primer pairs amplifying the 35S promoter oRG89 and the NOS-terminator oSH07 with adjacent CDS respectively. The correct sequence of PSTOL1 was reconfirmed by sequencing with the primer pair oKas4603 and oKas4604. The correct...

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